Rapid microbial DNA genome sequencing comparison detection and identification method

The invention discloses a rapid microbial DNA genome sequencing comparison detection and identification method, comprising the following steps: performing on-site DNA extraction at a microbial samplecollection site; performing quality inspection on DNA of a microbial sample; performing extremely long fragment sequencing on qualified DNA through a genetic sequencer; comparing sequenced genomic data; outputting a comparison result through a computer computing system; and reporting and summarizing the comparison report from the computer computing system. The method has the beneficial effects ofacquiring the accurate, rapid and timely bacterium identification results based on the whole genome of microorganisms, and can be widely applied to the rapid identification of bacteria in agricultureand hospitals. 种快速微生物DNA基因组测序比对检测鉴定的方法，包括以下步骤：在微生物样品采集地进行现场DNA抽提；对微生物样品的DNA进行质检；利用基因测序仪对质检合格的DNA进行超长片段测序；对测序出来的基因组数据进行比对；电脑运算系统将比对结果输出；电脑运算系统对比对结果作出报告总结。本发明的有益效果是基于微生物全基因组上准确、快速、时时的得到菌鉴定结果，可广泛的运用与农业和医院的菌体快速鉴定。