5'-nucleotidase activity detection method and reaction kinetic quantitative detector

The invention discloses a 5'-nucleotidase activity detection method and a reaction kinetic quantitative detector. The detector comprises: an excitation light source used for emitting excitation lights; a test rotating plate provided with a test sample pool and a control sample pool, wherein the test rotating plate is connected with a motor, the motor is connected with a controller, and the controller is respectively connected with the motor and the excitation light source and is used for controlling the excitation light source to emit the excitation lights; a beam splitter arranged on the optical path of the excitation light source and used for splitting the lights emitted by the excitation light source to a focusing mirror, wherein the focusing mirror is arranged on the split light path of the beam splitter and is used for focusing the lights transmitting the beam splitter, and the focused lights reach the test sample pool or the control sample pool; and a photomultiplier tube connected with the controller a