OCHROBACTRUM-MEDIATED TRANSFORMATION OF PLANTS

Methods and compositions for Ochrobactrum-mediated transformation of plants are provided. Methods include but are not limited to using an Ochrobactrum strain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include an Ochrobactrum strain, transfer DNAs, constructs and/or plasmids. These include Ochrobactrum strains having a plasmid comprising one or more virulence gene(s), border region, and/or origin of replication. Plant cells, tissues, plants, and seeds comprising a polynucleotide of interest produced by the methods are also provided. 本发明提供了用于苍白杆菌介导的植物转化的方法和组合物。这些方法包括但不限于使用苍白杆菌菌株将目的多核苷酸转移至植物细胞中。这些方法包括VirD2-依赖性方法。这些组合物包含苍白杆菌菌株、转移DNA、构建体和/或质粒。这些组合物包含具有包含个或多个毒力基因、边界区和/或复制起点的质粒的苍白杆菌菌株。本发明还提供了由这些方法产生的包含目的多核苷酸的植物细胞、组织、植物和种子。