Method for rapidly capturing and building database
The invention provides a method for rapidly capturing and building a database. By designing a DNA probe with the two ends provided with partial linker sequences and under the effect of CircLigase TM II ssDNA Ligase enzyme, a probe-target sequence complex, that is, a cyclized product can be formed be...
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| creator | YU YUEMEI SHAO QIANZHI HANG XINGYI WANG RUICHAO CAI WANSHI QU WUBIN |
| description | The invention provides a method for rapidly capturing and building a database. By designing a DNA probe with the two ends provided with partial linker sequences and under the effect of CircLigase TM II ssDNA Ligase enzyme, a probe-target sequence complex, that is, a cyclized product can be formed between the DNA probe and the target sequence which is completely complementary or partially complementary with the DNA probe. The cyclized product is subjected to index amplification under the effect of phi29 polymerase and a primer with identification, the amplified product generates a great amountof database suitable for an illumia sequencing platform under the effect of a pair of sequencing primers, compared with a traditional method for capturing and building the database, the preparation time for computer database is greatly shortened, and meanwhile, the tedious database preparation process and loss of effective database in the database preparation process are avoided.
通过设计两端带有部分接头序列的DNA探针，在CircLigaseII ssDNA L |
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通过设计两端带有部分接头序列的DNA探针，在CircLigaseII ssDNA L</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for rapidly capturing and building database |
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