Nano-antibody aiming at procalcitonin and application thereof
The invention relates to the technical field of biomedicines and clinical diagnosis and discloses a novel nano-antibody aiming at procalcitonin (PCT), a coding sequence of the novel nano-antibody andapplication of the novel nano-antibody to diagnosis. According to the novel nano-antibody provided by...
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| creator | LIU YAOFEI WANG BAOJUN LI LIANGHONG SHI SUJUAN WAN YAKUN FENG CHANGFANG TANG SHUANGSHUANG NIE ZHANGLIN ZHANG XIZHONG ZHENG DESEN |
| description | The invention relates to the technical field of biomedicines and clinical diagnosis and discloses a novel nano-antibody aiming at procalcitonin (PCT), a coding sequence of the novel nano-antibody andapplication of the novel nano-antibody to diagnosis. According to the novel nano-antibody provided by the invention, camels are immunized by utilizing the procalcitonin to obtain a high-quality nano-antibody gene library; then an enzyme-coupled plate is coated with the PCT to display a correct space structure of protein; the PCT is used as an antigen for screening a camel heavy-chain antibody bacteriophage display gene library, so that a nano-antibody gene aiming at the PCT is obtained; then the gene is transformed into escherichia coli and a nano-antibody strain which can be efficiently expressed in the escherichia coli is established. According to the novel nano-antibody provided by the invention, two different antigen epitopes, which can be combined with the nano-antibody, in the PCT are finally determined, and |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | Nano-antibody aiming at procalcitonin and application thereof |
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