Hepatitis B surface antigen chemiluminiscence immune detection reagent kit and application thereof

The invention relates to a hepatitis B surface antigen chemiluminiscence immune detection reagent kit and application thereof. The reagent kit comprises a magnetic particle reagent indirectly connected with an anti-HBsAg antibody 1, an HBsAg antibody 2 solution marked by a chemiluminiscence marker, a chemiluminiscence substrate solution and a cleaning solution; the anti-HBsAg antibody 1 at least comprises two antibodies or immune reaction fragments of antibodies, and at least comprises an anti-S-protein monoclonal antibody 1, the anti-HBsAg antibody 2 at least comprises two antibodies or immune reaction fragments of the antibodies and at least comprises an anti-S-protein monoclonal antibody 2, the epitopes of HBsAg corresponding to the anti-S-protein monoclonal antibody 1 and the anti-S-protein monoclonal antibody 2 are different, the reagent kit is the first case internationally and has the high sensitivity and accuracy during HBsAg detection, the HBsAg mutant strain detection capability is greatly improved,