Synchronous detection kit of fungi capable of producing trichothecene mycotoxins, fumonisins, and zearalenone
The invention discloses a synchronous detection kit of fungi capable of producing trichothecene mycotoxins, fumonisins, and zearalenone. The synchronous detection kit of fungi capable of producing trichothecene mycotoxins, fumonisins, and zearalenone is invented based multiple PCR detection, and com...
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| creator | NI DONG XIE XUEQIN CHEN JIAN LIN ZHU BAI ZEQING |
| description | The invention discloses a synchronous detection kit of fungi capable of producing trichothecene mycotoxins, fumonisins, and zearalenone. The synchronous detection kit of fungi capable of producing trichothecene mycotoxins, fumonisins, and zearalenone is invented based multiple PCR detection, and comprises a first primer pair, a second primer pair, a third primer pair, and an internal standard primer pair which are capable of realizing PCR reaction in a same reaction tube. A detection system disclosed in the invention is capable of realizing direct targeting at toxin synthesis essential genes,and is high in specificity; synchronous amplification of reference genes is realized, a positive control, a negative control, and a blank control are provided at the same time, so that false negativeand false positive are avoided effectively; and PCR index multiplication is capable of ensuring method sensitivity.
本发明公开了种产单端孢霉烯族毒素、伏马菌素和玉米赤霉烯酮真菌的同步检测试剂盒，基于多重PCR检测方法，包括能在同反应管中进行PCR反应的第引物对、第二引物对、第三引物对和内标引物对。本发明的检测体系直接靶向毒素合成必需基 |
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本发明公开了种产单端孢霉烯族毒素、伏马菌素和玉米赤霉烯酮真菌的同步检测试剂盒，基于多重PCR检测方法，包括能在同反应管中进行PCR反应的第引物对、第二引物对、第三引物对和内标引物对。本发明的检测体系直接靶向毒素合成必需基</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2018</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20180731&DB=EPODOC&CC=CN&NR=108342500A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20180731&DB=EPODOC&CC=CN&NR=108342500A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>NI DONG</creatorcontrib><creatorcontrib>XIE XUEQIN</creatorcontrib><creatorcontrib>CHEN JIAN</creatorcontrib><creatorcontrib>LIN ZHU</creatorcontrib><creatorcontrib>BAI ZEQING</creatorcontrib><title>Synchronous detection kit of fungi capable of producing trichothecene mycotoxins, fumonisins, and zearalenone</title><description>The invention discloses a synchronous detection kit of fungi capable of producing trichothecene mycotoxins, fumonisins, and zearalenone. The synchronous detection kit of fungi capable of producing trichothecene mycotoxins, fumonisins, and zearalenone is invented based multiple PCR detection, and comprises a first primer pair, a second primer pair, a third primer pair, and an internal standard primer pair which are capable of realizing PCR reaction in a same reaction tube. A detection system disclosed in the invention is capable of realizing direct targeting at toxin synthesis essential genes,and is high in specificity; synchronous amplification of reference genes is realized, a positive control, a negative control, and a blank control are provided at the same time, so that false negativeand false positive are avoided effectively; and PCR index multiplication is capable of ensuring method sensitivity.
本发明公开了种产单端孢霉烯族毒素、伏马菌素和玉米赤霉烯酮真菌的同步检测试剂盒，基于多重PCR检测方法，包括能在同反应管中进行PCR反应的第引物对、第二引物对、第三引物对和内标引物对。本发明的检测体系直接靶向毒素合成必需基</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2018</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjL0KwkAQhNNYiPoOa68Q_8BWgmJlo31YL5tkMdk97jZgfHqN-ABWM3zMN-Okvfbi6qCiXYSCjJyxCjzYQEsoO6kYHHq8NzQAH7ToHEsFFtjVajU5EoK2d2r6ZImLj9SqcPx2lAJehAEbEhWaJqMSm0izX06S-el4y85L8ppT9DicWZ5dVul-s13v0vSw-WfzBunlQvY</recordid><startdate>20180731</startdate><enddate>20180731</enddate><creator>NI DONG</creator><creator>XIE XUEQIN</creator><creator>CHEN JIAN</creator><creator>LIN ZHU</creator><creator>BAI ZEQING</creator><scope>EVB</scope></search><sort><creationdate>20180731</creationdate><title>Synchronous detection kit of fungi capable of producing trichothecene mycotoxins, fumonisins, and zearalenone</title><author>NI DONG ; XIE XUEQIN ; CHEN JIAN ; LIN ZHU ; BAI ZEQING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN108342500A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2018</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>NI DONG</creatorcontrib><creatorcontrib>XIE XUEQIN</creatorcontrib><creatorcontrib>CHEN JIAN</creatorcontrib><creatorcontrib>LIN ZHU</creatorcontrib><creatorcontrib>BAI ZEQING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>NI DONG</au><au>XIE XUEQIN</au><au>CHEN JIAN</au><au>LIN ZHU</au><au>BAI ZEQING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Synchronous detection kit of fungi capable of producing trichothecene mycotoxins, fumonisins, and zearalenone</title><date>2018-07-31</date><risdate>2018</risdate><abstract>The invention discloses a synchronous detection kit of fungi capable of producing trichothecene mycotoxins, fumonisins, and zearalenone. The synchronous detection kit of fungi capable of producing trichothecene mycotoxins, fumonisins, and zearalenone is invented based multiple PCR detection, and comprises a first primer pair, a second primer pair, a third primer pair, and an internal standard primer pair which are capable of realizing PCR reaction in a same reaction tube. A detection system disclosed in the invention is capable of realizing direct targeting at toxin synthesis essential genes,and is high in specificity; synchronous amplification of reference genes is realized, a positive control, a negative control, and a blank control are provided at the same time, so that false negativeand false positive are avoided effectively; and PCR index multiplication is capable of ensuring method sensitivity.
本发明公开了种产单端孢霉烯族毒素、伏马菌素和玉米赤霉烯酮真菌的同步检测试剂盒，基于多重PCR检测方法，包括能在同反应管中进行PCR反应的第引物对、第二引物对、第三引物对和内标引物对。本发明的检测体系直接靶向毒素合成必需基</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Synchronous detection kit of fungi capable of producing trichothecene mycotoxins, fumonisins, and zearalenone |
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