Diclofenac quantum dot immunochromatography detection card and detection method based on signal amplification system

The invention relates to a diclofenac quantum dot immunochromatography detection card and detection method based on a signal amplification system. The detection card comprises a lining plate, a detection antigen release pad, a quantum dot probe release pad, a chromatography film and a water absorpti...

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Bibliographische Detailangaben
Hauptverfasser: ZHONG RUIMIN, HUANG WANQIONG, ZHANG PENG, HUANG ZUANYUAN, ZHU ZHE, GUO JIEBIAO, WU LANJIE
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention relates to a diclofenac quantum dot immunochromatography detection card and detection method based on a signal amplification system. The detection card comprises a lining plate, a detection antigen release pad, a quantum dot probe release pad, a chromatography film and a water absorption pad, wherein the detection antigen release pad contains detection antigens formed by coupling ovalbumin and diclofenac; the quantum dot probe release pad comprises a quantum dot probe labeled with anti-ovalbumin antibodies; the chromatography film is provided with a detection line and a quality control line; the detection line is fixed with anti-diclofenac antibodies; the quality control line is fixed with ovalbumin antigens; the detection antigens are dissolved into the detection system fromthe release pad; a ternary system immune complex is formed with the detection antibodies and marking antibodies; fluorescence detection signals are generated. Under the detection reference effects ofthe quality control line,