Method for extracting, culturing and purifying amniotic stem cells

The invention discloses a method for extracting, culturing and purifying amniotic stem cells. The method comprises S1: material preparation: taking a normal placenta which is acquired in 4h after cesarean section in 38+/-1 weeks and has no hepatitis, syphilis, HIV and other infectious diseases, mechanically separating an amniotic membrane from the placenta, simultaneously, taking off a large areaof the amniotic membrane around the umbilical region, putting the amniotic membrane into a double-antibody-containing PBS container and repeatedly washing the amniotic membrane to remove blood and fragments, and S2: amniotic stem cell extraction: (1) directly adding the amniotic membrane pieces into a container containing an uniform mixture of 0.25% of trypsin and 0.02% of an EDTA digestion solution without cutting, putting the container into an incubator at 37 DEG C and carrying out treatment for 0.5h. The method separates, extracts, cultures and purifies the amniotic membrane pieces in the placenta tissue and carrie