Quad-fluorescence PCR method for detecting 5 animal-derived components
A quad-fluorescence PCR method for detecting 5 animal-derived components comprises four elements of sample DNA, primers and probes, a fluorescent PCR premixed liquid and a positive control; the primers and probes are designed according to specific genes of 5 animals comprising voles, rabbits, horses...
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| creator | DONG RUI XUN SIYING LIU TINGJU CHEN MEI SUN DUANFANG TIAN ZHIQIANG ZHANG QIAN LI CHUNYU |
| description | A quad-fluorescence PCR method for detecting 5 animal-derived components comprises four elements of sample DNA, primers and probes, a fluorescent PCR premixed liquid and a positive control; the primers and probes are designed according to specific genes of 5 animals comprising voles, rabbits, horses, donkeys and mules, target sequences are amplified and a fluorescence signal is stimulated, and non-target sequences are not amplified and have no fluorescence signal. The method comprises the steps: establishing the quad-fluorescence PCR primer and probe system for detecting the components derivedfrom the voles, rabbits, horses, donkeys and mules; and determining the quad-fluorescence PCR identification method for detecting the components derived from the voles, rabbits, horses, donkeys and mules. The method has the advantages of small standard error, short detection time, simultaneous detection of multiple target genes and reagent cost saving, and is suitable for identification of animalproducts.
检测5种动物源性成分的四重荧光 |
| format | Patent |
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检测5种动物源性成分的四重荧光</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Quad-fluorescence PCR method for detecting 5 animal-derived components |
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