Preparation method of moelleriella protoplast
The invention relates to a preparation method of moelleriella protoplast. Healthy and strong mycelia on a PDA thallus culture medium are inoculated into a PDB liquid culture medium; shake cultivationis performed for 36 to 54h at 150 rpm and 28 DEG C; then, the mycelia are filtered by four layers of...
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| creator | SUI JUNWEN NIU YAJING XIE YONGXIAO WEI XIUYAN YAO LINGDAN QIU JUNZHI XUE QINGLIN YANG FANG CHEN YUXI TU XINYA |
| description | The invention relates to a preparation method of moelleriella protoplast. Healthy and strong mycelia on a PDA thallus culture medium are inoculated into a PDB liquid culture medium; shake cultivationis performed for 36 to 54h at 150 rpm and 28 DEG C; then, the mycelia are filtered by four layers of sterile gauzes; a sterile mortar is used for grinding the mycelia; the ground mycelia are put intothe PDB culture medium to be subjected to shake cultivation for 16 h at 100 rpm and 28 DEG C. Then, the mycelia are filtered out by the four layers of sterile gauzes, then, the mycelia are transferredinto a sterile triangular bottle by a sterile medicine spoon; the mycelia is pretreated for 30 minutes by 10 ml of 0 to 0.08 percent beta-mercaptoethanol; 20 to 26.6 mg of fungal wall degrading enzymes (enzyme liquid pH is 5.6 to 6.2) are used for performing enzymolysis for 2.4 to 3.3h at 24 to 30 DEG C; when 0.4 to 0.7 mol/L of solution is used as an osmotic pressure stabilizing agent, the protoplast quantity reaches 2.2 |
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Healthy and strong mycelia on a PDA thallus culture medium are inoculated into a PDB liquid culture medium; shake cultivationis performed for 36 to 54h at 150 rpm and 28 DEG C; then, the mycelia are filtered by four layers of sterile gauzes; a sterile mortar is used for grinding the mycelia; the ground mycelia are put intothe PDB culture medium to be subjected to shake cultivation for 16 h at 100 rpm and 28 DEG C. 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Healthy and strong mycelia on a PDA thallus culture medium are inoculated into a PDB liquid culture medium; shake cultivationis performed for 36 to 54h at 150 rpm and 28 DEG C; then, the mycelia are filtered by four layers of sterile gauzes; a sterile mortar is used for grinding the mycelia; the ground mycelia are put intothe PDB culture medium to be subjected to shake cultivation for 16 h at 100 rpm and 28 DEG C. Then, the mycelia are filtered out by the four layers of sterile gauzes, then, the mycelia are transferredinto a sterile triangular bottle by a sterile medicine spoon; the mycelia is pretreated for 30 minutes by 10 ml of 0 to 0.08 percent beta-mercaptoethanol; 20 to 26.6 mg of fungal wall degrading enzymes (enzyme liquid pH is 5.6 to 6.2) are used for performing enzymolysis for 2.4 to 3.3h at 24 to 30 DEG C; when 0.4 to 0.7 mol/L of solution is used as an osmotic pressure stabilizing agent, the protoplast quantity reaches 2.2</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Preparation method of moelleriella protoplast |
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