Methods for sequencing part of DNA (Deoxyribonucleic Acid) of nuclear gene Hsp82 of brachionus rotifer and identifying brachionus rotifer

The invention relates to methods for sequencing part of DNA (Deoxyribonucleic Acid) of a nuclear gene Hsp82 of a brachionus rotifer and identifying the brachionus rotifer. The sequencing method comprises the following steps of extraction of total DNA of the brachionus rotifer, amplification with PCR (Polymerase Chain Reaction) primers and sequencing, and phylogenetic analysis; the identification method comprises the following steps of identifying by using the DNA of the nuclear gene Hsp82 of the brachionus rotifer. The PCR primers designed in the sequencing method can be used for amplifying the DNA of the nuclear gene Hsp82 of brachionus rubens and the like; a sequencing result can be used for accurately identifying the brachionus rubens and the like; the identification difficulty existingby utilizing morphology and an ultramicrostructure is overcome, and the possibility that populations compete, which is brought as the populations are miscellaneous, to cause the yield reduction and even the massive death of