Co-culture supernatant containing autologous CIK cells and application thereof
The invention discloses co-culture supernatant containing autologous CIK cells and application thereof, wherein the co-culture supernatant containing the autologous CIK cells is prepared by a following method. The specific method comprises the steps that: (1) the autogenous CIK cells are cultured un...
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| creator | LAI CONGXIAN MAI ZHIGUO XIAO XIULI YUAN JINGNA HE BINGKUN HE YUE LI WEIWEI LI JIEZHEN |
| description | The invention discloses co-culture supernatant containing autologous CIK cells and application thereof, wherein the co-culture supernatant containing the autologous CIK cells is prepared by a following method. The specific method comprises the steps that: (1) the autogenous CIK cells are cultured until the fourteenth day, and concentration of the autologous CIK cells reaches 1-5*10 cells/ml, and after centrifugation, supernatant 1 and bottom cells 1 are obtained respectively; (2) the bottom cells 1 are washed with normal saline and centrifuged to obtain bottom cells 2; (3) the supernatant 1obtained in step (1) is further centrifuged, and bottom flocculent is discarded to obtain supernatant 2; (4) the supernatant 2 of the step (3) and the bottom cells 2 of the step (2) are mixed, addedwith the normal saline, human albumin, and gentamicin sulfate to prepare cell suspension, namely, the co-culture supernatant containing autologous CIK cells. Natural cytokines synthesized and secretedby the autologous CIK cells a |
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The specific method comprises the steps that: (1) the autogenous CIK cells are cultured until the fourteenth day, and concentration of the autologous CIK cells reaches 1-5*10 cells/ml, and after centrifugation, supernatant 1 and bottom cells 1 are obtained respectively; (2) the bottom cells 1 are washed with normal saline and centrifuged to obtain bottom cells 2; (3) the supernatant 1obtained in step (1) is further centrifuged, and bottom flocculent is discarded to obtain supernatant 2; (4) the supernatant 2 of the step (3) and the bottom cells 2 of the step (2) are mixed, addedwith the normal saline, human albumin, and gentamicin sulfate to prepare cell suspension, namely, the co-culture supernatant containing autologous CIK cells. 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The specific method comprises the steps that: (1) the autogenous CIK cells are cultured until the fourteenth day, and concentration of the autologous CIK cells reaches 1-5*10 cells/ml, and after centrifugation, supernatant 1 and bottom cells 1 are obtained respectively; (2) the bottom cells 1 are washed with normal saline and centrifuged to obtain bottom cells 2; (3) the supernatant 1obtained in step (1) is further centrifuged, and bottom flocculent is discarded to obtain supernatant 2; (4) the supernatant 2 of the step (3) and the bottom cells 2 of the step (2) are mixed, addedwith the normal saline, human albumin, and gentamicin sulfate to prepare cell suspension, namely, the co-culture supernatant containing autologous CIK cells. 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The specific method comprises the steps that: (1) the autogenous CIK cells are cultured until the fourteenth day, and concentration of the autologous CIK cells reaches 1-5*10 cells/ml, and after centrifugation, supernatant 1 and bottom cells 1 are obtained respectively; (2) the bottom cells 1 are washed with normal saline and centrifuged to obtain bottom cells 2; (3) the supernatant 1obtained in step (1) is further centrifuged, and bottom flocculent is discarded to obtain supernatant 2; (4) the supernatant 2 of the step (3) and the bottom cells 2 of the step (2) are mixed, addedwith the normal saline, human albumin, and gentamicin sulfate to prepare cell suspension, namely, the co-culture supernatant containing autologous CIK cells. Natural cytokines synthesized and secretedby the autologous CIK cells a</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
| title | Co-culture supernatant containing autologous CIK cells and application thereof |
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