Co-culture supernatant containing autologous CIK cells and application thereof

The invention discloses co-culture supernatant containing autologous CIK cells and application thereof, wherein the co-culture supernatant containing the autologous CIK cells is prepared by a following method. The specific method comprises the steps that: (1) the autogenous CIK cells are cultured until the fourteenth day, and concentration of the autologous CIK cells reaches 1-5*10 cells/ml, and after centrifugation, supernatant 1 and bottom cells 1 are obtained respectively; (2) the bottom cells 1 are washed with normal saline and centrifuged to obtain bottom cells 2; (3) the supernatant 1obtained in step (1) is further centrifuged, and bottom flocculent is discarded to obtain supernatant 2; (4) the supernatant 2 of the step (3) and the bottom cells 2 of the step (2) are mixed, addedwith the normal saline, human albumin, and gentamicin sulfate to prepare cell suspension, namely, the co-culture supernatant containing autologous CIK cells. Natural cytokines synthesized and secretedby the autologous CIK cells a