Cross-linked starch biosynthesis recombinant gene YXI and application thereof

The invention discloses a cross-linked starch biosynthesis recombinant gene YXI and application thereof. The nucleotide sequence is shown as SEQ ID NO:1, glgC55 of the ADP-glucose pyrophosphorylase coding gene is separated out from a mutant strain of escherichia coli JM109-3, and an ADP-glucose pyrophosphorylase (AGPase) small subunit coding gene (SSU cDNA) and a starch grain-combined starch synthase coding gene GBSSI promoter are respectively separated out from potatoes. An AGPase subunit composed of 431 amino acids is coded by glgC55, wherein four specific mutations, which are K39E, V71A, I248V and K304E, exist, the first two appear in the Rossmann fold regions of the oligomeric subunit N-terminal metabolic groups, and the latter two appear in oligomeric subunit C-terminal heterogeneously regulated and oligomerized LbH groups. 本发明公开了种交联淀粉生物合成重组基因及其应用，其核苷酸序列如SEQ ID NO:1所示，从大肠杆菌JM109-3突变株中分离获得了腺苷二磷酸葡萄糖焦磷酸化酶编码基因的glgC55，从马铃薯分别分离获得了腺苷二磷酸葡萄糖焦磷酸化酶AGPase小亚基编码基因SSU cDNA和与淀粉粒结合的淀粉合成酶编码基因GBSSI启动子。其中，glgC55编码431个氨基酸组成的