Method for secretorily expressing glucose oxidase, and recombinant bacteria and applications thereof

The invention relates to a method for secretorily expressing glucose oxidase, and recombinant bacteria and applications thereof, and discloses a method capable of effectively promoting GOD expression and improving the enzyme activity. The method can be realized by co-expressing a GOD gene and a PDI1...

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Bibliographische Detailangaben
Hauptverfasser:	ZHUANG YINGPING, CHU JU, XIAO CIYING, QIAN JIANGCHAO, WEI DONGSHENG, WU FAN, ZHANG SILIANG, WANG ZEJIAN, DUAN GUANGDONG, LI LIANG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a method for secretorily expressing glucose oxidase, and recombinant bacteria and applications thereof, and discloses a method capable of effectively promoting GOD expression and improving the enzyme activity. The method can be realized by co-expressing a GOD gene and a PDI1 gene or a PDI3 gene in saccharomycetes. Furthermore, the invention further provides efficiently secreted recombinant bacteria constructed by taking Pichia pastoris as a host. 本发明涉及分泌表达葡萄糖氧化酶(GOD)的方法、重组菌及其应用，揭示种能够有效促进GOD表达、提高其酶活的方法，通过在酵母菌中共表达GOD基因和PDI1基因或PDI3基因来实现。同时，本发明还提供了以毕赤酵母(Pichia pastoris)为宿主构建的高效分泌GOD的重组菌。