Biological extraction preparation method of high-purity medicinal bone peptide solution

The invention discloses a biological extraction preparation method of a high-purity medicinal bone peptide solution. The method comprises specific operation steps as follows: limb bones of a pig are subjected to hot-pressing extraction, cold storage, fat removal, alcohol precipitation, concentration, acid, alkaline and neutral adjustment, 100 DEG C*30 min heating, secondary filtration, mixed protein removal, heat adsorption, ultrafiltration, multistage high-voltage electrophoresis separation steps for preparation of the biological extraction preparation method. According to the biological extraction preparation method of the high-purity medicinal bone peptide solution, the bone peptide solution has anti-inflammatory and pain relieving functions and is clinically applied to treatment of fracture healing, osteoporosis and hyperosteogeny and rheumatic arthritis. 本发明公开了种高纯药用骨肽溶液的生物提取制备方法，具体操作步骤为：将猪的四肢骨经热压提取，冷藏去脂肪、醇沉淀、浓缩、酸性碱性和中性100℃×30min加热二次过滤，除去杂蛋白、热吸附、超滤，多级高压电泳分离步骤制取高纯药用骨肽溶液；本发明是种高纯药用骨肽溶液的生物提取制备方法，所述骨肽溶液具有抗炎、镇痛