Monoclonal antibody of S-adenosylmethionine synthetase and application thereof

The invention discloses a monoclonal antibody of S-adenosylmethionine synthetase. A heavy chain variable region sequence of the monoclonal antibody contains CDR amino acid sequences shown by SEQ ID NO.2, 4 and 6; a light chain variable region sequence contains CDR amino acid sequences shown by SEQ I...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: WENG WEINING, XUN MENG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a monoclonal antibody of S-adenosylmethionine synthetase. A heavy chain variable region sequence of the monoclonal antibody contains CDR amino acid sequences shown by SEQ ID NO.2, 4 and 6; a light chain variable region sequence contains CDR amino acid sequences shown by SEQ ID NO.9, 11 and 13. The polypeptide of the enzyme is used as an immunogen for immunizing a mouse to obtain lymphocyte; a hybridoma fusion technology is used for preparing fusion cells; a cell strain capable of detecting and identifying the S-adenosylmethionine synthetase at high affinity and high specificity is obtained. The monoclonal antibody can be used for detecting the content of S-adenosylmethionine synthetase in bodies of animals and plants. The monoclonal antibody provides a targeted protein study tool, so that the deep level study of protein network atlas and the protein expression change on different plant types, different strains or different development periods can be realized. 本发明公开了种S-腺苷蛋氨酸合成酶的单克隆抗体,所述