Method for guiding helicobacter pylori to eradicate drug use based on PGM high throughput sequencing technology

The invention provides a method for guiding helicobacter pylori to eradicate drug use based on a PGM high throughput sequencing technology, and mainly aims to realize detection of helicobacter pylori on antibiotic drug resistance and host drug metabolic enzyme types. The method mainly comprises the...

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Hauptverfasser:	YANG NINGMIN, XU PEISONG, YANG BITE
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS SPIRITS VINEGAR WINE
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creator	YANG NINGMIN XU PEISONG YANG BITE
description	The invention provides a method for guiding helicobacter pylori to eradicate drug use based on a PGM high throughput sequencing technology, and mainly aims to realize detection of helicobacter pylori on antibiotic drug resistance and host drug metabolic enzyme types. The method mainly comprises the steps of designing a specific primer for covering VacA, 23SrRNA, gyrA, CYP2C192 and CYP2C193 gene to-be-detected regions to form a Primer Pool; extracting host and helicobacter pylori genome to perform amplification; performing PGM library preparation, purification, quantifying and sequencing; and working out drug resistance proportion and drug metabolic enzyme types. By adoption of multiple PCR technologies, synchronous amplification of multiple sites can be realized; and by combination with PGM sequencing, guiding to clinical detection and eradiation of the helicobacter pylori can be facilitated. 本发明提供了种基于PGM高通量测序技术指导幽门螺杆菌根除用药的方法，其主要是针对于幽门螺杆菌对抗生素的耐药性以及宿主药物代谢酶类型的检测。本方法主要包括：设计覆盖VacA、23SrRNA、gyrA、CYP2C19*2、CYP2C19
format	Patent
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title	Method for guiding helicobacter pylori to eradicate drug use based on PGM high throughput sequencing technology
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