Heparin sodium refining method

Heparin sodium refining method

The invention relates to the technical field of heparin sodium preparation and discloses a heparin sodium refining method. The heparin sodium refining method includes: dissolving crude heparin sodium into NaCl solution to obtain crude solution; sequentially subjecting the crude solution to twice enz...

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Hauptverfasser: CHEN JIANKE, LYU BAOJUN, CHEN GUANZHEN, CHEN ZONGRU, DONG YIBIN
Format: Patent
Sprache:chi ; eng
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CHEMISTRY
COMPOSITIONS BASED THEREON
DERIVATIVES THEREOF
METALLURGY
ORGANIC MACROMOLECULAR COMPOUNDS
POLYSACCHARIDES
THEIR PREPARATION OR CHEMICAL WORKING-UP
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creator CHEN JIANKE
LYU BAOJUN
CHEN GUANZHEN
CHEN ZONGRU
DONG YIBIN
description The invention relates to the technical field of heparin sodium preparation and discloses a heparin sodium refining method. The heparin sodium refining method includes: dissolving crude heparin sodium into NaCl solution to obtain crude solution; sequentially subjecting the crude solution to twice enzymolysis for enzymatic hydrolysate; adding ethyl alcohol into the enzymatic hydrolysate, stirring, standing, and filtering to obtain precipitates; completely dissolving the precipitates, and purifying through ion exchange columns to obtain elution liquid; decolorizing the elution liquid through absorption chromatograph columns to obtain heparin sodium solution. The heparin sodium refining method has advantages of simplicity in operation, short process time and high product yield, and low light absorptivity, high purity and high activity of products are realized. 本发明公开了种肝素钠的精制方法，涉及肝素钠的制备技术领域。肝素钠的精制方法包括：将粗品肝素钠溶解于NaCl溶液得到粗溶液；将所述粗溶液进行先后两次酶解得到酶解液；向所述酶解液中加入乙醇，经搅拌静置后过滤取沉淀；将所述沉淀完全溶解后上离子交换柱纯化得到洗脱液；将所述洗脱液上吸附层析柱脱色得到肝素钠溶液。本发明提
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The heparin sodium refining method includes: dissolving crude heparin sodium into NaCl solution to obtain crude solution; sequentially subjecting the crude solution to twice enzymolysis for enzymatic hydrolysate; adding ethyl alcohol into the enzymatic hydrolysate, stirring, standing, and filtering to obtain precipitates; completely dissolving the precipitates, and purifying through ion exchange columns to obtain elution liquid; decolorizing the elution liquid through absorption chromatograph columns to obtain heparin sodium solution. The heparin sodium refining method has advantages of simplicity in operation, short process time and high product yield, and low light absorptivity, high purity and high activity of products are realized. 本发明公开了种肝素钠的精制方法，涉及肝素钠的制备技术领域。肝素钠的精制方法包括：将粗品肝素钠溶解于NaCl溶液得到粗溶液；将所述粗溶液进行先后两次酶解得到酶解液；向所述酶解液中加入乙醇，经搅拌静置后过滤取沉淀；将所述沉淀完全溶解后上离子交换柱纯化得到洗脱液；将所述洗脱液上吸附层析柱脱色得到肝素钠溶液。本发明提</description><language>chi ; eng</language><subject>CHEMISTRY ; COMPOSITIONS BASED THEREON ; DERIVATIVES THEREOF ; METALLURGY ; ORGANIC MACROMOLECULAR COMPOUNDS ; POLYSACCHARIDES ; THEIR PREPARATION OR CHEMICAL WORKING-UP</subject><creationdate>2017</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20170818&amp;DB=EPODOC&amp;CC=CN&amp;NR=107056966A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20170818&amp;DB=EPODOC&amp;CC=CN&amp;NR=107056966A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CHEN JIANKE</creatorcontrib><creatorcontrib>LYU BAOJUN</creatorcontrib><creatorcontrib>CHEN GUANZHEN</creatorcontrib><creatorcontrib>CHEN ZONGRU</creatorcontrib><creatorcontrib>DONG YIBIN</creatorcontrib><title>Heparin sodium refining method</title><description>The invention relates to the technical field of heparin sodium preparation and discloses a heparin sodium refining method. 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subjects CHEMISTRY
COMPOSITIONS BASED THEREON
DERIVATIVES THEREOF
METALLURGY
ORGANIC MACROMOLECULAR COMPOUNDS
POLYSACCHARIDES
THEIR PREPARATION OR CHEMICAL WORKING-UP
title Heparin sodium refining method
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