Primer for detecting RT-PCR of Seneca Valley viruses and RT-PCR detecting method

The invention relates to the technical field of detection of pig viruses, in particular to a primer for detecting RT-PCR of Seneca Valley viruses and a detecting method. Nucleotide sequences of primers are as shown in SEQ ID NO: 1 and SEQ ID NO: 2. The RT-PCR detecting method comprises the following steps: designing and synthesizing the primers; extracting a sample RNA and storing for subsequent use; taking the obtained sample RNA as a template RNA, and carrying out RT-PCR reaction by using the primers; and judging and reading a detecting result. According to the scheme, the technical means of virus detection is enriched, specificity and sensitivity are good, the detection speed is high, and the application value is also high. 本发明涉及猪病毒的检测技术领域，具体涉及对塞尼卡谷病毒的RT-PCR检测引物及检测方法。所述引物的核苷酸序列如SEQ ID NO：1和SEQ ID NO：2所示；所述RT-PCR检测方法包括：设计合成所述的引物；提取样本RNA，保存备用；将所得的样本RNA作为模板RNA，利用所述引物进行RT-PCR反应；判读检测结果。本发明方案丰富了该病毒检测的技术手段，特异性和灵敏度好，检测速度快，具有很好的应用价值。