Wheat peptone industrial preparation method and application of wheat peptone

The invention relates to a wheat peptone industrial preparation method and an application of wheat peptone. The method includes the steps: firstly, taking wet-based wheat protein (wet gluten) as raw materials, efficiently crushing the raw materials, homogenizing the crushed raw materials, and performing hydrolysis on the homogenized raw materials by aid of a multi-enzyme system; secondly, filtering wheat protein enzymolysis liquid acquired through hydrolysis, performing stewing, injecting, killing enzyme and spray drying on the filtered wheat protein enzymolysis liquid to obtain wheat peptone. The protein content of the wheat peptone is 60-90%, the wheat peptone contains 18 kinds of amino acid, the content of glutamine/glutamic acid is larger than 25%, and the content of peptide segment with the molecular weight smaller than 1000Da is larger than 80%. The wheat peptone can completely or partially replace nitrogen sources in microorganism growth/fermentation media, is used for production of fermentation produc