Detection kit, primer and probe for simultaneously detecting and identifying porcine reproductive and respiratory syndrome and swine influenza

The invention discloses a detection kit, a primer and a probe for simultaneously detecting and identifying porcine reproductive and respiratory syndromes and swine influenza. The primer and the probe are shown as a sequence table SEQ ID NO: 1 to SEQ ID NO: 27. The invention further discloses the one...

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Hauptverfasser: ZHOU ZHI, LI YANXIN, WAN XIAONAN, ZHANG MOQI, LI BINGLING, QI WEI, HAO JUNHU, LIU QUANGUO, LIU YANHUA, SHI XIJU, HUAI SHUO, MA GUIPING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a detection kit, a primer and a probe for simultaneously detecting and identifying porcine reproductive and respiratory syndromes and swine influenza. The primer and the probe are shown as a sequence table SEQ ID NO: 1 to SEQ ID NO: 27. The invention further discloses the one-step-method multi-fluorescent RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection kit which is rapid, accurate and convenient to use and can be used for simultaneously detecting and identifying a North American type porcine reproductive and respiratory syndrome virus (PRRSV-NA), an European type porcine reproductive and respiratory syndrome virus (PRRSV-EU) and a swine influenza virus (SIV). A detection method disclosed by the invention can be used for realizing one-time sampling and one-time analysis and can realize the aim of simultaneously detecting and distinguishing three important swine diseases, so that the working amount and cost of detection are reduced, the detection of epidemic diseases c