Effective passage method for human diploid cell microcarrier culture
The invention relates to the technical field of biology, in particular to an effectively passage method for human diploid cell microcarrier culture in a bioreactor microcarrier scale-up culture process. According to the method, a microcarrier is utilized to culture a human diploid cell seed, a blank...
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| creator | WANG PING LI JIANBO GU CHUNYAN BAO JIAYUAN TANG CAIHUA ZHUANG FANGCHENG WU JIE ZHANG FENG JIANG YUNSHUI MAO ZI'AN MAO JIANGSEN |
| description | The invention relates to the technical field of biology, in particular to an effectively passage method for human diploid cell microcarrier culture in a bioreactor microcarrier scale-up culture process. According to the method, a microcarrier is utilized to culture a human diploid cell seed, a blank microcarrier is prebalanced in a seed culture system, a seed cell is digested by trypsin digestive juice containing sodium citrate, the seed cell is moderately stirred and cultured discontinuously for 3 to 6 hours in a seed reactor, and then the seed cell is transferred to an amplification reactor to be amplified and cultured to obtain effective passage in the human diploid cell microcarrier scale-up culture process. The effective passage method disclosed by the invention has effects of improving vigor, adherence rate and adherence evenness of cells in a passage process, obviously improving passage efficiency and solving the problem of difficulty in scale-up culture of the human diploid cells on the bioreactor and |
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According to the method, a microcarrier is utilized to culture a human diploid cell seed, a blank microcarrier is prebalanced in a seed culture system, a seed cell is digested by trypsin digestive juice containing sodium citrate, the seed cell is moderately stirred and cultured discontinuously for 3 to 6 hours in a seed reactor, and then the seed cell is transferred to an amplification reactor to be amplified and cultured to obtain effective passage in the human diploid cell microcarrier scale-up culture process. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Effective passage method for human diploid cell microcarrier culture |
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