Method for adherent culture of human diploid cells by means of carbon dioxide multi-layer cell culture flask

The invention provides a method for adherent culture of human diploid cells by means of a carbon dioxide multi-layer cell culture flask. By conducting adherent culture of human diploid cells by means of a multi-layer cell culture flask, spinner cultivation in the cell culture stage in a traditional viral vaccine preparing process can be replaced, labor intensity can be reduced, and occupied space and energy consumption can be reduced. Furthermore, the technique overcomes the defect that a current commercially available multi-layer cell culture flask with a vent cap is not suitable for a non-carbon dioxide dependent type culture environment, product input is reduced, and large-scale culture of human diploid cells can be better achieved. 本发明提供种过CO多层细胞培养瓶人二倍体细胞贴壁培养方法，利用多层细胞培养瓶进行人二倍体细胞贴壁培养，不仅可以替代传统病毒性疫苗制备工艺中细胞培养阶段的转瓶培养，降低劳动强度，减小占用空间和降低能源消耗，并且本发明的技术克服了目前市售具透气盖多层细胞培养瓶不适用于非二氧化碳依赖型培养环境的缺陷，降低了生产投入，更有利于人二倍体细胞的大规模培养。