LINKER ELEMENT AND METHOD OF USING SAME TO CONSTRUCT SEQUENCING LIBRARY
Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5&#...
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| creator | HE LINGYU JIANG HUI GENG ZHAO XIA FU SHUJIN ALEXEEV ANDREI JIANG YUAN SU XIAOSHAN WU FANZI LI YAQIAO ZHANG WENWEI DRMANAC RADOJE |
| description | Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5' end of the long strand has a phosphoric acid modification, and the 3' end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3' end thereof can be in a complementary pairing with the 5' end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.
提供了种接头元件和使用该接头元件构建测序文库的方法，所述接头元件由接头A和接头B组成；接头A由条核酸长链与条核酸短链互补配 |
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提供了种接头元件和使用该接头元件构建测序文库的方法，所述接头元件由接头A和接头B组成；接头A由条核酸长链与条核酸短链互补配</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMBINATORIAL CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2017</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170510&DB=EPODOC&CC=CN&NR=106661575A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,781,886,25568,76551</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170510&DB=EPODOC&CC=CN&NR=106661575A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HE LINGYU</creatorcontrib><creatorcontrib>JIANG HUI</creatorcontrib><creatorcontrib>GENG</creatorcontrib><creatorcontrib>ZHAO XIA</creatorcontrib><creatorcontrib>FU SHUJIN</creatorcontrib><creatorcontrib>ALEXEEV ANDREI</creatorcontrib><creatorcontrib>JIANG YUAN</creatorcontrib><creatorcontrib>SU XIAOSHAN</creatorcontrib><creatorcontrib>WU FANZI</creatorcontrib><creatorcontrib>LI YAQIAO</creatorcontrib><creatorcontrib>ZHANG WENWEI</creatorcontrib><creatorcontrib>DRMANAC RADOJE</creatorcontrib><title>LINKER ELEMENT AND METHOD OF USING SAME TO CONSTRUCT SEQUENCING LIBRARY</title><description>Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5' end of the long strand has a phosphoric acid modification, and the 3' end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3' end thereof can be in a complementary pairing with the 5' end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.
提供了种接头元件和使用该接头元件构建测序文库的方法，所述接头元件由接头A和接头B组成；接头A由条核酸长链与条核酸短链互补配</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMBINATORIAL CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2017</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZHD38fTzdg1ScPVx9XX1C1Fw9HNR8HUN8fB3UfB3UwgN9vRzVwh29HVVCPFXcPb3Cw4JCnUOUQh2DQx19XMGSfp4OgU5BkXyMLCmJeYUp_JCaW4GRTfXEGcP3dSC_PjU4oLE5NS81JJ4Zz9DAzMzM0NTc1NHY2LUAADKZyyL</recordid><startdate>20170510</startdate><enddate>20170510</enddate><creator>HE LINGYU</creator><creator>JIANG HUI</creator><creator>GENG</creator><creator>ZHAO XIA</creator><creator>FU SHUJIN</creator><creator>ALEXEEV ANDREI</creator><creator>JIANG YUAN</creator><creator>SU XIAOSHAN</creator><creator>WU FANZI</creator><creator>LI YAQIAO</creator><creator>ZHANG WENWEI</creator><creator>DRMANAC RADOJE</creator><scope>EVB</scope></search><sort><creationdate>20170510</creationdate><title>LINKER ELEMENT AND METHOD OF USING SAME TO CONSTRUCT SEQUENCING LIBRARY</title><author>HE LINGYU ; JIANG HUI ; GENG ; ZHAO XIA ; FU SHUJIN ; ALEXEEV ANDREI ; JIANG YUAN ; SU XIAOSHAN ; WU FANZI ; LI YAQIAO ; ZHANG WENWEI ; DRMANAC RADOJE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN106661575A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2017</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMBINATORIAL CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>HE LINGYU</creatorcontrib><creatorcontrib>JIANG HUI</creatorcontrib><creatorcontrib>GENG</creatorcontrib><creatorcontrib>ZHAO XIA</creatorcontrib><creatorcontrib>FU SHUJIN</creatorcontrib><creatorcontrib>ALEXEEV ANDREI</creatorcontrib><creatorcontrib>JIANG YUAN</creatorcontrib><creatorcontrib>SU XIAOSHAN</creatorcontrib><creatorcontrib>WU FANZI</creatorcontrib><creatorcontrib>LI YAQIAO</creatorcontrib><creatorcontrib>ZHANG WENWEI</creatorcontrib><creatorcontrib>DRMANAC RADOJE</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HE LINGYU</au><au>JIANG HUI</au><au>GENG</au><au>ZHAO XIA</au><au>FU SHUJIN</au><au>ALEXEEV ANDREI</au><au>JIANG YUAN</au><au>SU XIAOSHAN</au><au>WU FANZI</au><au>LI YAQIAO</au><au>ZHANG WENWEI</au><au>DRMANAC RADOJE</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>LINKER ELEMENT AND METHOD OF USING SAME TO CONSTRUCT SEQUENCING LIBRARY</title><date>2017-05-10</date><risdate>2017</risdate><abstract>Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5' end of the long strand has a phosphoric acid modification, and the 3' end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3' end thereof can be in a complementary pairing with the 5' end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.
提供了种接头元件和使用该接头元件构建测序文库的方法，所述接头元件由接头A和接头B组成；接头A由条核酸长链与条核酸短链互补配</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | LINKER ELEMENT AND METHOD OF USING SAME TO CONSTRUCT SEQUENCING LIBRARY |
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