Rapid chrysopa formosa brauer PCR detection based on specific SS-COI primers
The present invention provides a rapid chrysopa formosa brauer PCR detection based on specific SS-COI primers. The method comprises: preparing specific SS-COI primers, wherein a pair of specific SS-COI primers of chrysopa formosa brauer is designed according to the mitochondrial DNA sequence (genban...
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| creator | LI HAIQIANG A KEDAN DENG HAN LIU JIAN XU YAO DING RUIFENG LI HAOBIN PAN HONGSHENG WANG DONGMEI |
| description | The present invention provides a rapid chrysopa formosa brauer PCR detection based on specific SS-COI primers. The method comprises: preparing specific SS-COI primers, wherein a pair of specific SS-COI primers of chrysopa formosa brauer is designed according to the mitochondrial DNA sequence (genbank: KJ592501.1) of the chrysopa formosa brauer, and comprises the forward primer CfF5 (5'-TAGTTTAAGTTTATTAATTCGGG-3') and the reverse primer CfR1 (5'-TTGAAGATGCCAGTAATAAG-3'); extracting total DNA as a template; and carrying out a PCR amplification reaction by using the forward primer CfF5 and the reverse primer CfR1. According to the present invention, the chrysopa formosa brauer is detected by using the SS-COI PCR technology, wherein the technology has advantages of simple, rapid and efficient operation and high sensitivity, and the minimum DNA detection concentration of the chrysopa formosa brauer is 0.143 ng/[mu]L.
本发明提供了种基于特异性SS-COI引物的丽草蛉PCR快速检测方法，包括以下步骤：制备特异性SS-COI引物：根据丽草蛉的线粒体DNA序列（genbank:KJ592501.1），设计对丽草蛉的特 |
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本发明提供了种基于特异性SS-COI引物的丽草蛉PCR快速检测方法，包括以下步骤：制备特异性SS-COI引物：根据丽草蛉的线粒体DNA序列（genbank:KJ592501.1），设计对丽草蛉的特</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2017</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170503&DB=EPODOC&CC=CN&NR=106609306A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170503&DB=EPODOC&CC=CN&NR=106609306A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LI HAIQIANG</creatorcontrib><creatorcontrib>A KEDAN</creatorcontrib><creatorcontrib>DENG HAN</creatorcontrib><creatorcontrib>LIU JIAN</creatorcontrib><creatorcontrib>XU YAO</creatorcontrib><creatorcontrib>DING RUIFENG</creatorcontrib><creatorcontrib>LI HAOBIN</creatorcontrib><creatorcontrib>PAN HONGSHENG</creatorcontrib><creatorcontrib>WANG DONGMEI</creatorcontrib><title>Rapid chrysopa formosa brauer PCR detection based on specific SS-COI primers</title><description>The present invention provides a rapid chrysopa formosa brauer PCR detection based on specific SS-COI primers. The method comprises: preparing specific SS-COI primers, wherein a pair of specific SS-COI primers of chrysopa formosa brauer is designed according to the mitochondrial DNA sequence (genbank: KJ592501.1) of the chrysopa formosa brauer, and comprises the forward primer CfF5 (5'-TAGTTTAAGTTTATTAATTCGGG-3') and the reverse primer CfR1 (5'-TTGAAGATGCCAGTAATAAG-3'); extracting total DNA as a template; and carrying out a PCR amplification reaction by using the forward primer CfF5 and the reverse primer CfR1. According to the present invention, the chrysopa formosa brauer is detected by using the SS-COI PCR technology, wherein the technology has advantages of simple, rapid and efficient operation and high sensitivity, and the minimum DNA detection concentration of the chrysopa formosa brauer is 0.143 ng/[mu]L.
本发明提供了种基于特异性SS-COI引物的丽草蛉PCR快速检测方法，包括以下步骤：制备特异性SS-COI引物：根据丽草蛉的线粒体DNA序列（genbank:KJ592501.1），设计对丽草蛉的特</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2017</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZPAJSizITFFIziiqLM4vSFRIyy_KzS9OVEgqSixNLVIIcA5SSEktSU0uyczPU0hKLE5NUQAyigtSkzPTMpMVgoN1nf09FQqKMnNTi4p5GFjTEnOKU3mhNDeDoptriLOHbmpBfnxqcUFicmpeakm8s5-hgZmZgaWxgZmjMTFqACWdNLg</recordid><startdate>20170503</startdate><enddate>20170503</enddate><creator>LI HAIQIANG</creator><creator>A KEDAN</creator><creator>DENG HAN</creator><creator>LIU JIAN</creator><creator>XU YAO</creator><creator>DING RUIFENG</creator><creator>LI HAOBIN</creator><creator>PAN HONGSHENG</creator><creator>WANG DONGMEI</creator><scope>EVB</scope></search><sort><creationdate>20170503</creationdate><title>Rapid chrysopa formosa brauer PCR detection based on specific SS-COI primers</title><author>LI HAIQIANG ; A KEDAN ; DENG HAN ; LIU JIAN ; XU YAO ; DING RUIFENG ; LI HAOBIN ; PAN HONGSHENG ; WANG DONGMEI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN106609306A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2017</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LI HAIQIANG</creatorcontrib><creatorcontrib>A KEDAN</creatorcontrib><creatorcontrib>DENG HAN</creatorcontrib><creatorcontrib>LIU JIAN</creatorcontrib><creatorcontrib>XU YAO</creatorcontrib><creatorcontrib>DING RUIFENG</creatorcontrib><creatorcontrib>LI HAOBIN</creatorcontrib><creatorcontrib>PAN HONGSHENG</creatorcontrib><creatorcontrib>WANG DONGMEI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LI HAIQIANG</au><au>A KEDAN</au><au>DENG HAN</au><au>LIU JIAN</au><au>XU YAO</au><au>DING RUIFENG</au><au>LI HAOBIN</au><au>PAN HONGSHENG</au><au>WANG DONGMEI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Rapid chrysopa formosa brauer PCR detection based on specific SS-COI primers</title><date>2017-05-03</date><risdate>2017</risdate><abstract>The present invention provides a rapid chrysopa formosa brauer PCR detection based on specific SS-COI primers. The method comprises: preparing specific SS-COI primers, wherein a pair of specific SS-COI primers of chrysopa formosa brauer is designed according to the mitochondrial DNA sequence (genbank: KJ592501.1) of the chrysopa formosa brauer, and comprises the forward primer CfF5 (5'-TAGTTTAAGTTTATTAATTCGGG-3') and the reverse primer CfR1 (5'-TTGAAGATGCCAGTAATAAG-3'); extracting total DNA as a template; and carrying out a PCR amplification reaction by using the forward primer CfF5 and the reverse primer CfR1. According to the present invention, the chrysopa formosa brauer is detected by using the SS-COI PCR technology, wherein the technology has advantages of simple, rapid and efficient operation and high sensitivity, and the minimum DNA detection concentration of the chrysopa formosa brauer is 0.143 ng/[mu]L.
本发明提供了种基于特异性SS-COI引物的丽草蛉PCR快速检测方法，包括以下步骤：制备特异性SS-COI引物：根据丽草蛉的线粒体DNA序列（genbank:KJ592501.1），设计对丽草蛉的特</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Rapid chrysopa formosa brauer PCR detection based on specific SS-COI primers |
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