Establishing method of CD36 mutant gene stable eukaryotic expression cell line causing CD36 deletion

The invention provides an establishing method of a CD36 mutant gene stable eukaryotic expression cell line causing CD36 deletion. The method comprises a while process of extracting total RNA from a CD36 deletion individual and conducting reverse transcription so as to obtain CD36 cDNA of whole segment of a CD36 mutant gene open reading frame; establishing a recombinant cloning vector containing the CD36 mutant gene cDNA and an EGFP fluorescent reporter gene, transforming the recombinant cloning vector into escherichia coli, and conducting plasmid amplification and identification; and transfecting the recombinant cloning vector into CHO-K1 cells, and conducting screening and identification so as to obtain the CD36 mutant gene stable eukaryotic expression cell line. The CD36 is distributed in a plurality of cells and tissues, and CD36 encoding gene mutation can cause the CD36 deletion in the individual. The CD36 takes an important effect in various physiological and pathological processes, involving such diseas