Kit for rapidly classifying different groups of spinibarbus hollandi

The invention belongs to the technical field of aquatic product genetic classification, and particularly relates to a kit for rapidly classifying different groups of spinibarbus hollandi. The kit comprises taq enzyme premix liquid, distilled water and a primer, wherein the primer comprises a first r...

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Bibliographische Detailangaben
Hauptverfasser: GU PINGHUA, YANG YANG, SHU HU, LI QIANG, YUE LEI, LAN ZHAOJUN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention belongs to the technical field of aquatic product genetic classification, and particularly relates to a kit for rapidly classifying different groups of spinibarbus hollandi. The kit comprises taq enzyme premix liquid, distilled water and a primer, wherein the primer comprises a first reagent, a second reagent and a third reagent, and each of the first reagent, the second reagent and the third reagent is a mix reagent containing four kinds of SSR primers different in length. Through the kit, classification is easy and convenient; the SSR primers of different product lengths are mixed, so that the workload is greatly reduced in the operation process, and classification is more accurate; SSR molecular markers are used for rapid classification, and a batch of individuals can be classified as soon as possible within 2 hours. 本发明属于水产遗传分类技术领域,具体地说,对光倒刺鲃不同群体进行分类的试剂盒。所述的试剂盒包括taq酶预混液、蒸馏水、引物,所述的引物包括试剂1、试剂2和试剂3,试剂1、试剂2和试剂3为分别含有4种长度不同的SSR引物的混合试剂,使用该试剂盒进行分类操作简便,将数种产物长度不同的SSR进行混合,极大的减少了操作过程中的工作量,更为准确,使用SSR分子标记