Nucleotide mutational site for indicating high oleic acid content of rapeseeds

The invention discloses a nucleotide mutational site for indicating high oleic acid content of rapeseeds and relates to the field of plant molecular seed breeding. When any nucleotide mutational site exists in a certain variety (strain) of the rapeseeds, the variety (strain) has a high oleic acid co...

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Hauptverfasser: ZHANG JIEFU, PU HUIMING, HU MAOLONG, CHEN SONG, LONG WEIHUA, GAO JIANQIN, QI CUNKOU
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creator ZHANG JIEFU
PU HUIMING
HU MAOLONG
CHEN SONG
LONG WEIHUA
GAO JIANQIN
QI CUNKOU
description The invention discloses a nucleotide mutational site for indicating high oleic acid content of rapeseeds and relates to the field of plant molecular seed breeding. When any nucleotide mutational site exists in a certain variety (strain) of the rapeseeds, the variety (strain) has a high oleic acid content property; when two nucleotide mutational sites exist at the same time, higher oleic acid content is indicated. According to the nucleotide mutational site, one rape high oleic acid mutant is used as a material, and PCR (Polymerase Chain Reaction) amplification is carried out on a rape oleic acid desaturase gene (BnFAD2), so as to obtain two high oleic acid mutational sites in the mutant. The high oleic acid rape variety (strain) containing the mutational sites is hybridized with a common variety (strain), and the mutational sites are detected in a subsequent separation generation strain, and the rape variety (strain) with the oleic acid content which is 85 percent can be efficiently and accurately obtained. 本
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Nucleotide mutational site for indicating high oleic acid content of rapeseeds
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