Chamaecyparis pisifera cv.aurea subculture method

The invention discloses a chamaecyparis pisifera cv.aurea subculture method which comprises the following steps: (1) culture medium preparation: selecting a basic culture medium, adding sucrose and agar, then adding 6-benzylamino adenine and naphthylacetic acid, adjusting a pH value and sterilizing; (2) explant processing: cutting off the yellow part of a chamaecyparis pisifera cv.aurea stem section in initiation culture and cleaning; (3) inoculated culture: cutting off an old wound of an explant obtained in the step (2), inserting into the culture medium obtained in the step (1) and putting in a culture chamber for culture. Compared with the prior art, the chamaecyparis pisifera cv.aurea subculture method disclosed by the invention not only has the advantage of tissue culture and propagation in the prior art, but also can (1) obviously improve the proliferation multiple of subculture to 2 times or above through specific culture medium, hormone type and concentration in combination with other methods, (2) inc