Laying hen oviduct ampulla epithelial cell culture and oxidative stress model establishment method

The invention discloses a laying hen oviduct ampulla epithelial cell culture and oxidative stress model establishment method. The cell culture method consists of: soaking a whole section of laying hen oviduct in PBS, and removing mesentery, connective tissue and blood; cutting off the oviduct ampulla, performing cleaning and then cutting it into tissue pieces; transferring the cleaned tissue pieces into collagenase IV for digestion; filtering the digestive juice, centrifuging the filtrate and discarding the supernate, adding a complete medium to resuspend the cell, and finally transferring the cell into a culture bottle to conduct cultivation. The oxidative stress model establishment method consists of: testing the heavy metal of different concentrations and the cell viability under different action time, and determining the action time point; designing different heavy metal concentration gradients according to the time point, determining cell apoptosis and other conditions, and establishing an oxidative stre