Conjugate comprising erythropoietin and a branched polymer structure

The invention relates to: a conjugate comprising erythropoietin (EPO) and an asymmetric branched polymer structure having two monomethoxy-poly(ethylene glycol) (mPEG) branches, wherein the molecular mass of one of said mPEG branches is between 10 kDa and 14 kDa, and the molecular mass of the other mPEG branch is between 17 kDa and 23 kDa; and pharmaceutical compositions containing said conjugate. The invention also relates to a method for producing PEGylated EPO, characterised in that said protein is conjugated to an asymmetric branched polymer structure, having two mPEG branches with the above-mentioned molecular masses. 本发明公开了包含促红细胞生成素(EPO)和含有两个单甲氧基聚乙二醇(mPEG)分枝的不对称支化聚合物结构的缀合物，其中所述mPEG分枝之的分子量在10kDa和14kDa之间，和另mPEG分枝的分子量在17kDa和23kDa之间；以及公开了包含该缀合物的药物组合物。本发明还提供了用于获得经PEG化的EPO的方法，其特征在于，将所述蛋白质缀合至具有两个有着上述分子量的mPEG分枝的不对称支化聚合物结构。