Method for building agrobacterium-mediated sempervivum-tectorum efficient transfection system
The invention discloses a method for building an agrobacterium-mediated sempervivum-tectorum efficient transfection system. The method includes the following steps of infecting and co-culturing, wherein sempervivum-tectorum aseptic seedling leaf blocks are put into a triangular flask, agrobacterium...
Gespeichert in:
| Hauptverfasser: | , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | SHENG CHENGYE CHEN HAITAO DONG YANG ZHANG YESHENG |
| description | The invention discloses a method for building an agrobacterium-mediated sempervivum-tectorum efficient transfection system. The method includes the following steps of infecting and co-culturing, wherein sempervivum-tectorum aseptic seedling leaf blocks are put into a triangular flask, agrobacterium liquid is poured, an opening of the triangular flask is sealed and infected, the agrobacterium liquid is poured out after infection, leaves are aired and transplanted into a co-culture medium and subjected to dark culture, and the callus tissue is obtained; screening, wherein the co-cultured callus tissue is transplanted into a screening culture medium to screened two times, and the buds are obtained; resistance seedling culturing, wherein the obtained buds are transplanted into a resistance seedling culture medium and cultured, and the resistance seedlings are obtained; positive seedling detecting. According to the method, sempervivum tectorum is converted in an agrobacterium-mediated mode, the sempervivum-tectoru |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN106119277A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN106119277A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN106119277A3</originalsourceid><addsrcrecordid>eNqNyjsKwkAURuE0FqLu4bqAgFEwWEpQbLSylTCZ-SdeyDyYuQm4e1O4AKsDH2dZvO6QdzBkQ6Ju5MGw70n1KXRKCxKPrnQwrASGMlxEmniaUaAlpNERrGXN8EKSlM92dg6e8icL3LpYWDVkbH5dFdvr5dncSsTQIkel4SFt86h2x6o67ev6fPjn-QKpfD2y</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Method for building agrobacterium-mediated sempervivum-tectorum efficient transfection system</title><source>esp@cenet</source><creator>SHENG CHENGYE ; CHEN HAITAO ; DONG YANG ; ZHANG YESHENG</creator><creatorcontrib>SHENG CHENGYE ; CHEN HAITAO ; DONG YANG ; ZHANG YESHENG</creatorcontrib><description>The invention discloses a method for building an agrobacterium-mediated sempervivum-tectorum efficient transfection system. The method includes the following steps of infecting and co-culturing, wherein sempervivum-tectorum aseptic seedling leaf blocks are put into a triangular flask, agrobacterium liquid is poured, an opening of the triangular flask is sealed and infected, the agrobacterium liquid is poured out after infection, leaves are aired and transplanted into a co-culture medium and subjected to dark culture, and the callus tissue is obtained; screening, wherein the co-cultured callus tissue is transplanted into a screening culture medium to screened two times, and the buds are obtained; resistance seedling culturing, wherein the obtained buds are transplanted into a resistance seedling culture medium and cultured, and the resistance seedlings are obtained; positive seedling detecting. According to the method, sempervivum tectorum is converted in an agrobacterium-mediated mode, the sempervivum-tectoru</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TRAPPING ; VINEGAR ; WINE</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20161116&DB=EPODOC&CC=CN&NR=106119277A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20161116&DB=EPODOC&CC=CN&NR=106119277A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SHENG CHENGYE</creatorcontrib><creatorcontrib>CHEN HAITAO</creatorcontrib><creatorcontrib>DONG YANG</creatorcontrib><creatorcontrib>ZHANG YESHENG</creatorcontrib><title>Method for building agrobacterium-mediated sempervivum-tectorum efficient transfection system</title><description>The invention discloses a method for building an agrobacterium-mediated sempervivum-tectorum efficient transfection system. The method includes the following steps of infecting and co-culturing, wherein sempervivum-tectorum aseptic seedling leaf blocks are put into a triangular flask, agrobacterium liquid is poured, an opening of the triangular flask is sealed and infected, the agrobacterium liquid is poured out after infection, leaves are aired and transplanted into a co-culture medium and subjected to dark culture, and the callus tissue is obtained; screening, wherein the co-cultured callus tissue is transplanted into a screening culture medium to screened two times, and the buds are obtained; resistance seedling culturing, wherein the obtained buds are transplanted into a resistance seedling culture medium and cultured, and the resistance seedlings are obtained; positive seedling detecting. According to the method, sempervivum tectorum is converted in an agrobacterium-mediated mode, the sempervivum-tectoru</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2016</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyjsKwkAURuE0FqLu4bqAgFEwWEpQbLSylTCZ-SdeyDyYuQm4e1O4AKsDH2dZvO6QdzBkQ6Ju5MGw70n1KXRKCxKPrnQwrASGMlxEmniaUaAlpNERrGXN8EKSlM92dg6e8icL3LpYWDVkbH5dFdvr5dncSsTQIkel4SFt86h2x6o67ev6fPjn-QKpfD2y</recordid><startdate>20161116</startdate><enddate>20161116</enddate><creator>SHENG CHENGYE</creator><creator>CHEN HAITAO</creator><creator>DONG YANG</creator><creator>ZHANG YESHENG</creator><scope>EVB</scope></search><sort><creationdate>20161116</creationdate><title>Method for building agrobacterium-mediated sempervivum-tectorum efficient transfection system</title><author>SHENG CHENGYE ; CHEN HAITAO ; DONG YANG ; ZHANG YESHENG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN106119277A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2016</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TRAPPING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>SHENG CHENGYE</creatorcontrib><creatorcontrib>CHEN HAITAO</creatorcontrib><creatorcontrib>DONG YANG</creatorcontrib><creatorcontrib>ZHANG YESHENG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>SHENG CHENGYE</au><au>CHEN HAITAO</au><au>DONG YANG</au><au>ZHANG YESHENG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for building agrobacterium-mediated sempervivum-tectorum efficient transfection system</title><date>2016-11-16</date><risdate>2016</risdate><abstract>The invention discloses a method for building an agrobacterium-mediated sempervivum-tectorum efficient transfection system. The method includes the following steps of infecting and co-culturing, wherein sempervivum-tectorum aseptic seedling leaf blocks are put into a triangular flask, agrobacterium liquid is poured, an opening of the triangular flask is sealed and infected, the agrobacterium liquid is poured out after infection, leaves are aired and transplanted into a co-culture medium and subjected to dark culture, and the callus tissue is obtained; screening, wherein the co-cultured callus tissue is transplanted into a screening culture medium to screened two times, and the buds are obtained; resistance seedling culturing, wherein the obtained buds are transplanted into a resistance seedling culture medium and cultured, and the resistance seedlings are obtained; positive seedling detecting. According to the method, sempervivum tectorum is converted in an agrobacterium-mediated mode, the sempervivum-tectoru</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN106119277A |
| source | esp@cenet |
| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
| title | Method for building agrobacterium-mediated sempervivum-tectorum efficient transfection system |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T22%3A45%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=SHENG%20CHENGYE&rft.date=2016-11-16&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN106119277A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |