Method for building agrobacterium-mediated sempervivum-tectorum efficient transfection system

The invention discloses a method for building an agrobacterium-mediated sempervivum-tectorum efficient transfection system. The method includes the following steps of infecting and co-culturing, wherein sempervivum-tectorum aseptic seedling leaf blocks are put into a triangular flask, agrobacterium liquid is poured, an opening of the triangular flask is sealed and infected, the agrobacterium liquid is poured out after infection, leaves are aired and transplanted into a co-culture medium and subjected to dark culture, and the callus tissue is obtained; screening, wherein the co-cultured callus tissue is transplanted into a screening culture medium to screened two times, and the buds are obtained; resistance seedling culturing, wherein the obtained buds are transplanted into a resistance seedling culture medium and cultured, and the resistance seedlings are obtained; positive seedling detecting. According to the method, sempervivum tectorum is converted in an agrobacterium-mediated mode, the sempervivum-tectoru