RECOMBINANT DEAMIDATED GLIADIN ANTIGEN
The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the anti...
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| creator | LU YABIN DESAI URVEE WALKER ROGER SHAN DAMING |
| description | The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.
本发明提供种通过将来自受试者的体液样品与固定在固体支持物上的，由麦醇溶蛋白融合蛋白六聚体形成的抗原接触，确定受试者是否患有乳糜泻的方法。所述抗原的麦醇溶蛋白融合蛋白包括连接于标签(比如谷胱甘肽-S转移酶(GST)蛋白的重组脱酰胺化麦醇溶蛋白。通过将麦醇溶蛋白融合蛋白固定于固体支持物制备所述抗原。所述抗原可以进步包括与麦醇溶蛋白融合蛋白交联的组织转谷氨酰胺酶(tTG)。当tTG存在时，在固定于固相之前混合所述tTG和所述重组脱酰胺化麦醇溶蛋白。 |
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本发明提供种通过将来自受试者的体液样品与固定在固体支持物上的，由麦醇溶蛋白融合蛋白六聚体形成的抗原接触，确定受试者是否患有乳糜泻的方法。所述抗原的麦醇溶蛋白融合蛋白包括连接于标签(比如谷胱甘肽-S转移酶(GST)蛋白的重组脱酰胺化麦醇溶蛋白。通过将麦醇溶蛋白融合蛋白固定于固体支持物制备所述抗原。所述抗原可以进步包括与麦醇溶蛋白融合蛋白交联的组织转谷氨酰胺酶(tTG)。当tTG存在时，在固定于固相之前混合所述tTG和所述重组脱酰胺化麦醇溶蛋白。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PHYSICS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20161116&DB=EPODOC&CC=CN&NR=106117330A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20161116&DB=EPODOC&CC=CN&NR=106117330A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LU YABIN</creatorcontrib><creatorcontrib>DESAI URVEE</creatorcontrib><creatorcontrib>WALKER ROGER</creatorcontrib><creatorcontrib>SHAN DAMING</creatorcontrib><title>RECOMBINANT DEAMIDATED GLIADIN ANTIGEN</title><description>The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.
本发明提供种通过将来自受试者的体液样品与固定在固体支持物上的，由麦醇溶蛋白融合蛋白六聚体形成的抗原接触，确定受试者是否患有乳糜泻的方法。所述抗原的麦醇溶蛋白融合蛋白包括连接于标签(比如谷胱甘肽-S转移酶(GST)蛋白的重组脱酰胺化麦醇溶蛋白。通过将麦醇溶蛋白融合蛋白固定于固体支持物制备所述抗原。所述抗原可以进步包括与麦醇溶蛋白融合蛋白交联的组织转谷氨酰胺酶(tTG)。当tTG存在时，在固定于固相之前混合所述tTG和所述重组脱酰胺化麦醇溶蛋白。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PHYSICS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2016</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZFALcnX293Xy9HP0C1FwcXX09XRxDHF1UXD38XR08fRTAAp7urv68TCwpiXmFKfyQmluBkU31xBnD93Ugvz41OKCxOTUvNSSeGc_QwMzQ0NzY2MDR2Ni1AAAsAojSw</recordid><startdate>20161116</startdate><enddate>20161116</enddate><creator>LU YABIN</creator><creator>DESAI URVEE</creator><creator>WALKER ROGER</creator><creator>SHAN DAMING</creator><scope>EVB</scope></search><sort><creationdate>20161116</creationdate><title>RECOMBINANT DEAMIDATED GLIADIN ANTIGEN</title><author>LU YABIN ; DESAI URVEE ; WALKER ROGER ; SHAN DAMING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN106117330A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2016</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PHYSICS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LU YABIN</creatorcontrib><creatorcontrib>DESAI URVEE</creatorcontrib><creatorcontrib>WALKER ROGER</creatorcontrib><creatorcontrib>SHAN DAMING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LU YABIN</au><au>DESAI URVEE</au><au>WALKER ROGER</au><au>SHAN DAMING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>RECOMBINANT DEAMIDATED GLIADIN ANTIGEN</title><date>2016-11-16</date><risdate>2016</risdate><abstract>The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.
本发明提供种通过将来自受试者的体液样品与固定在固体支持物上的，由麦醇溶蛋白融合蛋白六聚体形成的抗原接触，确定受试者是否患有乳糜泻的方法。所述抗原的麦醇溶蛋白融合蛋白包括连接于标签(比如谷胱甘肽-S转移酶(GST)蛋白的重组脱酰胺化麦醇溶蛋白。通过将麦醇溶蛋白融合蛋白固定于固体支持物制备所述抗原。所述抗原可以进步包括与麦醇溶蛋白融合蛋白交联的组织转谷氨酰胺酶(tTG)。当tTG存在时，在固定于固相之前混合所述tTG和所述重组脱酰胺化麦醇溶蛋白。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | RECOMBINANT DEAMIDATED GLIADIN ANTIGEN |
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