Solution for improving hybridization efficiency of pseudomonas aeruginosa and pseudomonas aeruginosa hybridization method
The invention discloses a solution for improving hybridization efficiency of pseudomonas aeruginosa. The solution is characterized in that a solution composed of glycerin with the concentration being 10% and 0.05 M CaCl2 and a solution composed of glycerin with the concentration being 15% and 0.1 M...
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| creator | FAN ZEZHENG WANG XIAOQIAN GUO ZISHENG CHEN LIN |
| description | The invention discloses a solution for improving hybridization efficiency of pseudomonas aeruginosa. The solution is characterized in that a solution composed of glycerin with the concentration being 10% and 0.05 M CaCl2 and a solution composed of glycerin with the concentration being 15% and 0.1 M CaCl2 are selected to serve as solutions for improving hybridization efficiency, and the solution of 15% glycerin and 0.1 M CaCl2 is proper. Efficiency of exogenous genes entering recipient cells is changed through different physiological conditions. It is not easy to obtain a high-efficiency recombinant through laboratory conditions, and the formula and the ratio of a hybridization solution are determined on the basis. The efficiency in the bacterial hybridization process can be improved by means of the solution, and more recombinants can be obtained.
本发明公开了提高铜绿假单胞菌杂交效率的溶液，其特征在于：选择浓度范围10％甘油+0.05M CaCl、15％甘油+0.1M CaCl作为提高杂交效率的溶液，15％甘油+0.1M CaCl溶液为宜。本发明通过生理条件的不同改变外源基因进入受体细胞的效率。在实验室条件要想获得高效率的重组体并不是非常的容易，在此基础上进行了杂交溶液的 |
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本发明公开了提高铜绿假单胞菌杂交效率的溶液，其特征在于：选择浓度范围10％甘油+0.05M CaCl、15％甘油+0.1M CaCl作为提高杂交效率的溶液，15％甘油+0.1M CaCl溶液为宜。本发明通过生理条件的不同改变外源基因进入受体细胞的效率。在实验室条件要想获得高效率的重组体并不是非常的容易，在此基础上进行了杂交溶液的</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20161012&DB=EPODOC&CC=CN&NR=106011166A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,778,883,25551,76302</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20161012&DB=EPODOC&CC=CN&NR=106011166A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>FAN ZEZHENG</creatorcontrib><creatorcontrib>WANG XIAOQIAN</creatorcontrib><creatorcontrib>GUO ZISHENG</creatorcontrib><creatorcontrib>CHEN LIN</creatorcontrib><title>Solution for improving hybridization efficiency of pseudomonas aeruginosa and pseudomonas aeruginosa hybridization method</title><description>The invention discloses a solution for improving hybridization efficiency of pseudomonas aeruginosa. The solution is characterized in that a solution composed of glycerin with the concentration being 10% and 0.05 M CaCl2 and a solution composed of glycerin with the concentration being 15% and 0.1 M CaCl2 are selected to serve as solutions for improving hybridization efficiency, and the solution of 15% glycerin and 0.1 M CaCl2 is proper. Efficiency of exogenous genes entering recipient cells is changed through different physiological conditions. It is not easy to obtain a high-efficiency recombinant through laboratory conditions, and the formula and the ratio of a hybridization solution are determined on the basis. The efficiency in the bacterial hybridization process can be improved by means of the solution, and more recombinants can be obtained.
本发明公开了提高铜绿假单胞菌杂交效率的溶液，其特征在于：选择浓度范围10％甘油+0.05M CaCl、15％甘油+0.1M CaCl作为提高杂交效率的溶液，15％甘油+0.1M CaCl溶液为宜。本发明通过生理条件的不同改变外源基因进入受体细胞的效率。在实验室条件要想获得高效率的重组体并不是非常的容易，在此基础上进行了杂交溶液的</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2016</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjLEKwjAQQLM4iPoP5wcIDUJ3KYqTi-7lbC7tQXMXkkaoXy-Ii4Pg9Ib3eEszX3UsE6uA1wQcYtIHSw_DfE_s-IlvR95zxyTdDOohZipOgwpmQEqlZ9GMgOJ-qe9boGlQtzYLj2OmzYcrsz0db815R1FbyhE7Epra5mKrurLW1vVh_0_zAjPoSKE</recordid><startdate>20161012</startdate><enddate>20161012</enddate><creator>FAN ZEZHENG</creator><creator>WANG XIAOQIAN</creator><creator>GUO ZISHENG</creator><creator>CHEN LIN</creator><scope>EVB</scope></search><sort><creationdate>20161012</creationdate><title>Solution for improving hybridization efficiency of pseudomonas aeruginosa and pseudomonas aeruginosa hybridization method</title><author>FAN ZEZHENG ; WANG XIAOQIAN ; GUO ZISHENG ; CHEN LIN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN106011166A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2016</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>FAN ZEZHENG</creatorcontrib><creatorcontrib>WANG XIAOQIAN</creatorcontrib><creatorcontrib>GUO ZISHENG</creatorcontrib><creatorcontrib>CHEN LIN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>FAN ZEZHENG</au><au>WANG XIAOQIAN</au><au>GUO ZISHENG</au><au>CHEN LIN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Solution for improving hybridization efficiency of pseudomonas aeruginosa and pseudomonas aeruginosa hybridization method</title><date>2016-10-12</date><risdate>2016</risdate><abstract>The invention discloses a solution for improving hybridization efficiency of pseudomonas aeruginosa. The solution is characterized in that a solution composed of glycerin with the concentration being 10% and 0.05 M CaCl2 and a solution composed of glycerin with the concentration being 15% and 0.1 M CaCl2 are selected to serve as solutions for improving hybridization efficiency, and the solution of 15% glycerin and 0.1 M CaCl2 is proper. Efficiency of exogenous genes entering recipient cells is changed through different physiological conditions. It is not easy to obtain a high-efficiency recombinant through laboratory conditions, and the formula and the ratio of a hybridization solution are determined on the basis. The efficiency in the bacterial hybridization process can be improved by means of the solution, and more recombinants can be obtained.
本发明公开了提高铜绿假单胞菌杂交效率的溶液，其特征在于：选择浓度范围10％甘油+0.05M CaCl、15％甘油+0.1M CaCl作为提高杂交效率的溶液，15％甘油+0.1M CaCl溶液为宜。本发明通过生理条件的不同改变外源基因进入受体细胞的效率。在实验室条件要想获得高效率的重组体并不是非常的容易，在此基础上进行了杂交溶液的</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Solution for improving hybridization efficiency of pseudomonas aeruginosa and pseudomonas aeruginosa hybridization method |
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