Solution for improving hybridization efficiency of pseudomonas aeruginosa and pseudomonas aeruginosa hybridization method

The invention discloses a solution for improving hybridization efficiency of pseudomonas aeruginosa. The solution is characterized in that a solution composed of glycerin with the concentration being 10% and 0.05 M CaCl2 and a solution composed of glycerin with the concentration being 15% and 0.1 M CaCl2 are selected to serve as solutions for improving hybridization efficiency, and the solution of 15% glycerin and 0.1 M CaCl2 is proper. Efficiency of exogenous genes entering recipient cells is changed through different physiological conditions. It is not easy to obtain a high-efficiency recombinant through laboratory conditions, and the formula and the ratio of a hybridization solution are determined on the basis. The efficiency in the bacterial hybridization process can be improved by means of the solution, and more recombinants can be obtained. 本发明公开了提高铜绿假单胞菌杂交效率的溶液，其特征在于：选择浓度范围10％甘油+0.05M CaCl、15％甘油+0.1M CaCl作为提高杂交效率的溶液，15％甘油+0.1M CaCl溶液为宜。本发明通过生理条件的不同改变外源基因进入受体细胞的效率。在实验室条件要想获得高效率的重组体并不是非常的容易，在此基础上进行了杂交溶液的