Babesia canis PCR detection kit containing internal amplification control and use method of Babesia canis PCR detection kit
The invention provides a detection kit for detecting whether sick dogs are infected by Babesia and particularly relates to a Babesia canis PCR detection kit containing internal amplification control. The detection kit comprises a forward primer SEQ ID NO: 1, a reverse primer SEQ ID NO: 2, an interna...
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| creator | Yang Deji Xiao Xia Hong Yufang Yang Lun Yao Taiwei Zhao Yanbing Xu Jiarong |
| description | The invention provides a detection kit for detecting whether sick dogs are infected by Babesia and particularly relates to a Babesia canis PCR detection kit containing internal amplification control. The detection kit comprises a forward primer SEQ ID NO: 1, a reverse primer SEQ ID NO: 2, an internal amplification control plasmid pMD-IAC, a standard positive template plasmid pMD-18S rRNA, a standard negative template, a blank template, PCR reaction liquid 2*Taq PCR Mix and sterile water. The detection kit has main characteristics that the internal amplification control is added into a Babesia canis PCR detection system, a false negative phenomenon can be indicated, the false negative result caused when inhibitor exists in the reaction system, PCR instruments malfunction, the reaction system malfunctions, polymerase is inactivated, and the like can be eliminated, and the accuracy of the detection result is further improved.
本发明提供种检测患病犬是否感染巴贝斯虫的检测试剂盒，特别是含扩增内标的犬巴贝斯虫PCR检测试剂盒。该检测试剂盒包括上下游引物SEQ ID NO：1和SEQ ID NO：2，扩 |
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本发明提供种检测患病犬是否感染巴贝斯虫的检测试剂盒，特别是含扩增内标的犬巴贝斯虫PCR检测试剂盒。该检测试剂盒包括上下游引物SEQ ID NO：1和SEQ ID NO：2，扩</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160817&DB=EPODOC&CC=CN&NR=105861651A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160817&DB=EPODOC&CC=CN&NR=105861651A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Yang Deji</creatorcontrib><creatorcontrib>Xiao Xia</creatorcontrib><creatorcontrib>Hong Yufang</creatorcontrib><creatorcontrib>Yang Lun</creatorcontrib><creatorcontrib>Yao Taiwei</creatorcontrib><creatorcontrib>Zhao Yanbing</creatorcontrib><creatorcontrib>Xu Jiarong</creatorcontrib><title>Babesia canis PCR detection kit containing internal amplification control and use method of Babesia canis PCR detection kit</title><description>The invention provides a detection kit for detecting whether sick dogs are infected by Babesia and particularly relates to a Babesia canis PCR detection kit containing internal amplification control. The detection kit comprises a forward primer SEQ ID NO: 1, a reverse primer SEQ ID NO: 2, an internal amplification control plasmid pMD-IAC, a standard positive template plasmid pMD-18S rRNA, a standard negative template, a blank template, PCR reaction liquid 2*Taq PCR Mix and sterile water. The detection kit has main characteristics that the internal amplification control is added into a Babesia canis PCR detection system, a false negative phenomenon can be indicated, the false negative result caused when inhibitor exists in the reaction system, PCR instruments malfunction, the reaction system malfunctions, polymerase is inactivated, and the like can be eliminated, and the accuracy of the detection result is further improved.
本发明提供种检测患病犬是否感染巴贝斯虫的检测试剂盒，特别是含扩增内标的犬巴贝斯虫PCR检测试剂盒。该检测试剂盒包括上下游引物SEQ ID NO：1和SEQ ID NO：2，扩</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2016</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNzLsKAjEQheE0FqK-w_gAgkF2sdWgWImI_TImszqYnYTNWPnyXrDX6sDPxxmaxxrPVBjBo3CBgztCICWvnARurOCTKLKwXIBFqReMgF2O3LLHj3qLPr2qBLgXgo70mgKkFn58j82gxVho8t2RmW43J7ebUU4NlYyehLRxezuvlrWtK7ta_GOeKplGhA</recordid><startdate>20160817</startdate><enddate>20160817</enddate><creator>Yang Deji</creator><creator>Xiao Xia</creator><creator>Hong Yufang</creator><creator>Yang Lun</creator><creator>Yao Taiwei</creator><creator>Zhao Yanbing</creator><creator>Xu Jiarong</creator><scope>EVB</scope></search><sort><creationdate>20160817</creationdate><title>Babesia canis PCR detection kit containing internal amplification control and use method of Babesia canis PCR detection kit</title><author>Yang Deji ; Xiao Xia ; Hong Yufang ; Yang Lun ; Yao Taiwei ; Zhao Yanbing ; Xu Jiarong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN105861651A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2016</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>Yang Deji</creatorcontrib><creatorcontrib>Xiao Xia</creatorcontrib><creatorcontrib>Hong Yufang</creatorcontrib><creatorcontrib>Yang Lun</creatorcontrib><creatorcontrib>Yao Taiwei</creatorcontrib><creatorcontrib>Zhao Yanbing</creatorcontrib><creatorcontrib>Xu Jiarong</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Yang Deji</au><au>Xiao Xia</au><au>Hong Yufang</au><au>Yang Lun</au><au>Yao Taiwei</au><au>Zhao Yanbing</au><au>Xu Jiarong</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Babesia canis PCR detection kit containing internal amplification control and use method of Babesia canis PCR detection kit</title><date>2016-08-17</date><risdate>2016</risdate><abstract>The invention provides a detection kit for detecting whether sick dogs are infected by Babesia and particularly relates to a Babesia canis PCR detection kit containing internal amplification control. The detection kit comprises a forward primer SEQ ID NO: 1, a reverse primer SEQ ID NO: 2, an internal amplification control plasmid pMD-IAC, a standard positive template plasmid pMD-18S rRNA, a standard negative template, a blank template, PCR reaction liquid 2*Taq PCR Mix and sterile water. The detection kit has main characteristics that the internal amplification control is added into a Babesia canis PCR detection system, a false negative phenomenon can be indicated, the false negative result caused when inhibitor exists in the reaction system, PCR instruments malfunction, the reaction system malfunctions, polymerase is inactivated, and the like can be eliminated, and the accuracy of the detection result is further improved.
本发明提供种检测患病犬是否感染巴贝斯虫的检测试剂盒，特别是含扩增内标的犬巴贝斯虫PCR检测试剂盒。该检测试剂盒包括上下游引物SEQ ID NO：1和SEQ ID NO：2，扩</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Babesia canis PCR detection kit containing internal amplification control and use method of Babesia canis PCR detection kit |
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