Two-photon fluorescent probe as well as preparation method and application thereof
The invention discloses a two-photon fluorescent probe as well as a preparation method and application thereof. The two-photon fluorescent probe takes coumarin and rhodamine as a matrix. The structure of the two-photon fluorescent probe is described in the specification. Molecules of the two-photon...
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| creator | Shao Zonglong Wei Xinjie Meng Xiangming Shu Manzhou |
| description | The invention discloses a two-photon fluorescent probe as well as a preparation method and application thereof. The two-photon fluorescent probe takes coumarin and rhodamine as a matrix. The structure of the two-photon fluorescent probe is described in the specification. Molecules of the two-photon fluorescent probe show relatively high selectivity and sensitivity in a system in which palladium ions (II) and other positive ions coexist. A cytotoxicity test shows that the two-photon fluorescent probe disclosed by the invention has hardly any toxic effect on cells, and a two-photon fluorescence microscopy imaging experiment shows that the two-photon fluorescent probe has good HeLa cell permeability and is applicable to detection of distribution of palladium ions (II) in cells.
本发明公开了种双光子荧光探针及其制备方法和用途，其中双光子荧光探针是以香豆素与罗丹明为母体，其结构由以下表示：本发明荧光探针分子在钯离子(Ⅱ)与其他阳离子共存的体系中，表现出较高的选择性和灵敏性。细胞毒性测试表明本发明对细胞几乎没有毒性作用，双光子荧光显微成像实验表明本发明对HeLa细胞渗透性好，适于细胞内检测钯离子(Ⅱ)的分布。 |
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本发明公开了种双光子荧光探针及其制备方法和用途，其中双光子荧光探针是以香豆素与罗丹明为母体，其结构由以下表示：本发明荧光探针分子在钯离子(Ⅱ)与其他阳离子共存的体系中，表现出较高的选择性和灵敏性。细胞毒性测试表明本发明对细胞几乎没有毒性作用，双光子荧光显微成像实验表明本发明对HeLa细胞渗透性好，适于细胞内检测钯离子(Ⅱ)的分布。</description><language>chi ; eng</language><subject>ADHESIVES ; CHEMISTRY ; DYES ; HETEROCYCLIC COMPOUNDS ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE ; MEASURING ; METALLURGY ; MISCELLANEOUS APPLICATIONS OF MATERIALS ; MISCELLANEOUS COMPOSITIONS ; NATURAL RESINS ; ORGANIC CHEMISTRY ; PAINTS ; PHYSICS ; POLISHES ; TESTING</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160817&DB=EPODOC&CC=CN&NR=105859733A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76318</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160817&DB=EPODOC&CC=CN&NR=105859733A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Shao Zonglong</creatorcontrib><creatorcontrib>Wei Xinjie</creatorcontrib><creatorcontrib>Meng Xiangming</creatorcontrib><creatorcontrib>Shu Manzhou</creatorcontrib><title>Two-photon fluorescent probe as well as preparation method and application thereof</title><description>The invention discloses a two-photon fluorescent probe as well as a preparation method and application thereof. The two-photon fluorescent probe takes coumarin and rhodamine as a matrix. The structure of the two-photon fluorescent probe is described in the specification. Molecules of the two-photon fluorescent probe show relatively high selectivity and sensitivity in a system in which palladium ions (II) and other positive ions coexist. A cytotoxicity test shows that the two-photon fluorescent probe disclosed by the invention has hardly any toxic effect on cells, and a two-photon fluorescence microscopy imaging experiment shows that the two-photon fluorescent probe has good HeLa cell permeability and is applicable to detection of distribution of palladium ions (II) in cells.
本发明公开了种双光子荧光探针及其制备方法和用途，其中双光子荧光探针是以香豆素与罗丹明为母体，其结构由以下表示：本发明荧光探针分子在钯离子(Ⅱ)与其他阳离子共存的体系中，表现出较高的选择性和灵敏性。细胞毒性测试表明本发明对细胞几乎没有毒性作用，双光子荧光显微成像实验表明本发明对HeLa细胞渗透性好，适于细胞内检测钯离子(Ⅱ)的分布。</description><subject>ADHESIVES</subject><subject>CHEMISTRY</subject><subject>DYES</subject><subject>HETEROCYCLIC COMPOUNDS</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE</subject><subject>MEASURING</subject><subject>METALLURGY</subject><subject>MISCELLANEOUS APPLICATIONS OF MATERIALS</subject><subject>MISCELLANEOUS COMPOSITIONS</subject><subject>NATURAL RESINS</subject><subject>ORGANIC CHEMISTRY</subject><subject>PAINTS</subject><subject>PHYSICS</subject><subject>POLISHES</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2016</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZAgKKc_XLcjIL8nPU0jLKc0vSi1OTs0rUSgoyk9KVUgsVihPzckB0QVFqQWJRYklmUCFuaklGfkpCol5QFxQkJOZDBEuyUgtSs1P42FgTUvMKU7lhdLcDIpuriHOHrqpBfnxqcUFiUALUkvinf0MDUwtTC3NjY0djYlRAwBLRTiC</recordid><startdate>20160817</startdate><enddate>20160817</enddate><creator>Shao Zonglong</creator><creator>Wei Xinjie</creator><creator>Meng Xiangming</creator><creator>Shu Manzhou</creator><scope>EVB</scope></search><sort><creationdate>20160817</creationdate><title>Two-photon fluorescent probe as well as preparation method and application thereof</title><author>Shao Zonglong ; Wei Xinjie ; Meng Xiangming ; Shu Manzhou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN105859733A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2016</creationdate><topic>ADHESIVES</topic><topic>CHEMISTRY</topic><topic>DYES</topic><topic>HETEROCYCLIC COMPOUNDS</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE</topic><topic>MEASURING</topic><topic>METALLURGY</topic><topic>MISCELLANEOUS APPLICATIONS OF MATERIALS</topic><topic>MISCELLANEOUS COMPOSITIONS</topic><topic>NATURAL RESINS</topic><topic>ORGANIC CHEMISTRY</topic><topic>PAINTS</topic><topic>PHYSICS</topic><topic>POLISHES</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>Shao Zonglong</creatorcontrib><creatorcontrib>Wei Xinjie</creatorcontrib><creatorcontrib>Meng Xiangming</creatorcontrib><creatorcontrib>Shu Manzhou</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Shao Zonglong</au><au>Wei Xinjie</au><au>Meng Xiangming</au><au>Shu Manzhou</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Two-photon fluorescent probe as well as preparation method and application thereof</title><date>2016-08-17</date><risdate>2016</risdate><abstract>The invention discloses a two-photon fluorescent probe as well as a preparation method and application thereof. The two-photon fluorescent probe takes coumarin and rhodamine as a matrix. The structure of the two-photon fluorescent probe is described in the specification. Molecules of the two-photon fluorescent probe show relatively high selectivity and sensitivity in a system in which palladium ions (II) and other positive ions coexist. A cytotoxicity test shows that the two-photon fluorescent probe disclosed by the invention has hardly any toxic effect on cells, and a two-photon fluorescence microscopy imaging experiment shows that the two-photon fluorescent probe has good HeLa cell permeability and is applicable to detection of distribution of palladium ions (II) in cells.
本发明公开了种双光子荧光探针及其制备方法和用途，其中双光子荧光探针是以香豆素与罗丹明为母体，其结构由以下表示：本发明荧光探针分子在钯离子(Ⅱ)与其他阳离子共存的体系中，表现出较高的选择性和灵敏性。细胞毒性测试表明本发明对细胞几乎没有毒性作用，双光子荧光显微成像实验表明本发明对HeLa细胞渗透性好，适于细胞内检测钯离子(Ⅱ)的分布。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | ADHESIVES CHEMISTRY DYES HETEROCYCLIC COMPOUNDS INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE MEASURING METALLURGY MISCELLANEOUS APPLICATIONS OF MATERIALS MISCELLANEOUS COMPOSITIONS NATURAL RESINS ORGANIC CHEMISTRY PAINTS PHYSICS POLISHES TESTING |
| title | Two-photon fluorescent probe as well as preparation method and application thereof |
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