Two-photon fluorescent probe as well as preparation method and application thereof

The invention discloses a two-photon fluorescent probe as well as a preparation method and application thereof. The two-photon fluorescent probe takes coumarin and rhodamine as a matrix. The structure of the two-photon fluorescent probe is described in the specification. Molecules of the two-photon fluorescent probe show relatively high selectivity and sensitivity in a system in which palladium ions (II) and other positive ions coexist. A cytotoxicity test shows that the two-photon fluorescent probe disclosed by the invention has hardly any toxic effect on cells, and a two-photon fluorescence microscopy imaging experiment shows that the two-photon fluorescent probe has good HeLa cell permeability and is applicable to detection of distribution of palladium ions (II) in cells. 本发明公开了种双光子荧光探针及其制备方法和用途，其中双光子荧光探针是以香豆素与罗丹明为母体，其结构由以下表示：本发明荧光探针分子在钯离子(Ⅱ)与其他阳离子共存的体系中，表现出较高的选择性和灵敏性。细胞毒性测试表明本发明对细胞几乎没有毒性作用，双光子荧光显微成像实验表明本发明对HeLa细胞渗透性好，适于细胞内检测钯离子(Ⅱ)的分布。