Application of radix cyathulae saponin A in preparation of medicine for protecting human umbilical vein vascular endothelial cells

The invention discloses natural monomer radix cyathulae saponin A which has an effect of protecting homocysteine induced human umbilical vein vascular endothelial cell damage. Human umbilical vein vascular endothelial cell damage is induced by pretreatment of homocysteine having a certain concentration to construct a damage model; and radix cyathulae saponin A is added based on the model, and the effective concentration of the radix cyathulae saponin A for protecting the cells can be determined by cell viability detection. The radix cyathulae saponin A is applied to protection of human umbilical vein vascular endothelial cell for the first time. 本发明公开了种天然单体川牛膝皂苷A对同型半胱氨酸诱导的人脐静脉血管内皮细胞损伤的保护作用。通过定浓度的同型半胱氨酸预处理，诱导人脐静脉血管内皮细胞损伤，建立损伤模型。在此模型的基础上，加入川牛膝皂苷A，通过细胞活力检测，确定了川牛膝皂苷A保护细胞有效浓度。本发明首次应用川牛膝皂苷A对人脐静脉血管内皮细胞进行保护。