Extraction method of bovine tendon collagen
The invention provides an extraction method of bovine tendon collagen. The extraction method has the advantages of high yield, short production period and high product purity, and comprises the following steps of: preprocessing bovine tendon, and freezing and crushing the bovine tendon; putting the...
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| creator | HUANG HONGBAO ZHAO JING HUANG FACAN ZHENG DENGZHONG |
| description | The invention provides an extraction method of bovine tendon collagen. The extraction method has the advantages of high yield, short production period and high product purity, and comprises the following steps of: preprocessing bovine tendon, and freezing and crushing the bovine tendon; putting the crushed bovine tendon into a Tris-HCL buffer solution; then, adding cold-adapted protease MCP-01 accounting for 0.1 percent of the weight of the bovine tendon into the buffer solution; performing enzymolysis at 40 DEG C; then, obtaining an enzymolysis solution; centrifuging the enzymolysis solution; taking precipitates; dissolving the precipitates by 0.1-percent acetic acid; performing filtration to obtain filter liquid; salting out the filter liquid to obtain a salting-out solution; performing centrifugation twice; adding water for dissolution; then, adding 0.1-percent acetic acid for continuous dissolution; and performing freeze drying to obtain the bovine tendon collagen. The extraction method has the beneficial |
| format | Patent |
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The extraction method has the advantages of high yield, short production period and high product purity, and comprises the following steps of: preprocessing bovine tendon, and freezing and crushing the bovine tendon; putting the crushed bovine tendon into a Tris-HCL buffer solution; then, adding cold-adapted protease MCP-01 accounting for 0.1 percent of the weight of the bovine tendon into the buffer solution; performing enzymolysis at 40 DEG C; then, obtaining an enzymolysis solution; centrifuging the enzymolysis solution; taking precipitates; dissolving the precipitates by 0.1-percent acetic acid; performing filtration to obtain filter liquid; salting out the filter liquid to obtain a salting-out solution; performing centrifugation twice; adding water for dissolution; then, adding 0.1-percent acetic acid for continuous dissolution; and performing freeze drying to obtain the bovine tendon collagen. The extraction method has the beneficial</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160427&DB=EPODOC&CC=CN&NR=105524965A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76516</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160427&DB=EPODOC&CC=CN&NR=105524965A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HUANG HONGBAO</creatorcontrib><creatorcontrib>ZHAO JING</creatorcontrib><creatorcontrib>HUANG FACAN</creatorcontrib><creatorcontrib>ZHENG DENGZHONG</creatorcontrib><title>Extraction method of bovine tendon collagen</title><description>The invention provides an extraction method of bovine tendon collagen. The extraction method has the advantages of high yield, short production period and high product purity, and comprises the following steps of: preprocessing bovine tendon, and freezing and crushing the bovine tendon; putting the crushed bovine tendon into a Tris-HCL buffer solution; then, adding cold-adapted protease MCP-01 accounting for 0.1 percent of the weight of the bovine tendon into the buffer solution; performing enzymolysis at 40 DEG C; then, obtaining an enzymolysis solution; centrifuging the enzymolysis solution; taking precipitates; dissolving the precipitates by 0.1-percent acetic acid; performing filtration to obtain filter liquid; salting out the filter liquid to obtain a salting-out solution; performing centrifugation twice; adding water for dissolution; then, adding 0.1-percent acetic acid for continuous dissolution; and performing freeze drying to obtain the bovine tendon collagen. 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The extraction method has the advantages of high yield, short production period and high product purity, and comprises the following steps of: preprocessing bovine tendon, and freezing and crushing the bovine tendon; putting the crushed bovine tendon into a Tris-HCL buffer solution; then, adding cold-adapted protease MCP-01 accounting for 0.1 percent of the weight of the bovine tendon into the buffer solution; performing enzymolysis at 40 DEG C; then, obtaining an enzymolysis solution; centrifuging the enzymolysis solution; taking precipitates; dissolving the precipitates by 0.1-percent acetic acid; performing filtration to obtain filter liquid; salting out the filter liquid to obtain a salting-out solution; performing centrifugation twice; adding water for dissolution; then, adding 0.1-percent acetic acid for continuous dissolution; and performing freeze drying to obtain the bovine tendon collagen. The extraction method has the beneficial</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES SPIRITS VINEGAR WINE |
| title | Extraction method of bovine tendon collagen |
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