Microscale ultraviolet spectrophotometer quality detection method and kit

The invention provides a microscale ultraviolet spectrophotometer quality detection method and kit. The method creatively utilizes a series of salmon sperm DNA standard substances to realize quality examination of a microscale ultraviolet spectrophotometer. The method has high sensitivity, can detec...

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Bibliographische Detailangaben
Hauptverfasser: GAO YUNHUA, SUI ZHIWEI, WU LIQING, DONG LIANHUA, SHENG LINGHUI, CUI WEIQUN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention provides a microscale ultraviolet spectrophotometer quality detection method and kit. The method creatively utilizes a series of salmon sperm DNA standard substances to realize quality examination of a microscale ultraviolet spectrophotometer. The method has high sensitivity, can detect 10.7ng of DNA, has a wide linear range, and can detect DNA with content of 0-2163ng/microliter, wherein the range comprises four orders of magnitudes and comprises the existing microscale ultraviolet spectrophotometer measurement range on the market. A ultraviolet spectrophotometer national standard device-based test proves that the salmon sperm DNA standard substance has high accuracy. The method solves the problem that the microscale ultraviolet spectrophotometer has only a detection window, cannot receive a cuvette, cannot adjust wavelength and cannot be subjected to calibration according to the existing spectrophotometer detection national standards.