Preparation method of improved potato dextrose agar medium
The invention discloses a preparation method of an improved potato dextrose agar medium. The preparation method comprises the following steps: washing fresh potatoes, soaking potato blocks by bromoethane for 96h-108h in a closed container at the temperature of 25+/-1 DEG C, at the relative humidity...
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| creator | YANG WEIHONG LYU XINGPING CAO QINGGUO SONG JINYAO TONG BIN CHEN GENG ZHANG XIAOHUA |
| description | The invention discloses a preparation method of an improved potato dextrose agar medium. The preparation method comprises the following steps: washing fresh potatoes, soaking potato blocks by bromoethane for 96h-108h in a closed container at the temperature of 25+/-1 DEG C, at the relative humidity of 85% and at the absence of light, opening the container for ventilating for 30 min or more, weighing the processed potato blocks, adding water into the potato blocks and boiling for 10 min, filtering by adopting double-layer gauze, supplementing with water up to the original water amount, respectively adding glucose and agar, and carrying out sterilization after complete melting. The preparation method is simple and practical, the culturing speed of the PDA medium for fungi is obviously accelerated, and thus the improved potato dextrose medium has a wide promotional practical value. |
| format | Patent |
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The preparation method comprises the following steps: washing fresh potatoes, soaking potato blocks by bromoethane for 96h-108h in a closed container at the temperature of 25+/-1 DEG C, at the relative humidity of 85% and at the absence of light, opening the container for ventilating for 30 min or more, weighing the processed potato blocks, adding water into the potato blocks and boiling for 10 min, filtering by adopting double-layer gauze, supplementing with water up to the original water amount, respectively adding glucose and agar, and carrying out sterilization after complete melting. 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The preparation method comprises the following steps: washing fresh potatoes, soaking potato blocks by bromoethane for 96h-108h in a closed container at the temperature of 25+/-1 DEG C, at the relative humidity of 85% and at the absence of light, opening the container for ventilating for 30 min or more, weighing the processed potato blocks, adding water into the potato blocks and boiling for 10 min, filtering by adopting double-layer gauze, supplementing with water up to the original water amount, respectively adding glucose and agar, and carrying out sterilization after complete melting. The preparation method is simple and practical, the culturing speed of the PDA medium for fungi is obviously accelerated, and thus the improved potato dextrose medium has a wide promotional practical value.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Preparation method of improved potato dextrose agar medium |
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