Scallop species specificity detection primer and application

The invention belongs to the field of biotechnology and particularly relates to a scallop species specificity detection primer and application. The sequence of the scallop species specificity detection primer at the upper stream is 5'-agattcgggtatttggtgc-3', and the sequence of the scallop...

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Bibliographische Detailangaben
Hauptverfasser:	WAN CHAO, XU FENGMIN, SONG DAHE, LIU SHUYAN
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the field of biotechnology and particularly relates to a scallop species specificity detection primer and application. The sequence of the scallop species specificity detection primer at the upper stream is 5'-agattcgggtatttggtgc-3', and the sequence of the scallop species specificity detection primer at the down stream is 5'-cggtaaagtagaaacgggtat-3'. According to the application of the scallop species specificity detection primer, the scallop species specificity detection primer is used for PCR amplification of CoxI genes of scallops, a specificity electrophoretic band with the size of 129 bp can be generated through agarose gel electrophoresis after PCR amplification, and thus specificity identification is carried out on scallop ingredients. The specificity detection primer is reasonable in design and has good specificity and high detection sensitivity when used for scallop species detection, the scallop ingredients can be accurately identified simply through PCR analysis, and good specificity is achieved.