Method for identifying alfalfa brown spot
The invention discloses a method for identifying alfalfa brown spot. The method comprises the following steps: separating pathogens to be placed on a PDA culture medium platform to be cultured, washing with sterilized water, and filtering to obtain a spore suspending liquid; taking leaves of alfalfa...
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| creator | QU SHANMIN LIU XIANGPING LI GUOLIANG YANG ZHIMING |
| description | The invention discloses a method for identifying alfalfa brown spot. The method comprises the following steps: separating pathogens to be placed on a PDA culture medium platform to be cultured, washing with sterilized water, and filtering to obtain a spore suspending liquid; taking leaves of alfalfa, removing leafstalks, washing, conducting sterilization treatment, chopping into small blocks, putting into a 0.3% water agar culture medium containing 50 [mu]m/mL benzimidazole, and culturing for 4 days; selecting good leaves with the normal growth state, tiling on the culture medium, lightly stabbing the leaf surfaces with a pin, dripping the obtained spore suspending liquid at the stabbed parts, and conducting inoculation at the condition that the relative humidity is almost 100%, the temperature is 20 DEG C, and illumination is not provided, for 20 h; after the inoculation is finished, transferring the leaves into a growth box for culture, alternating the light intensity of 9000 lux and darkness every 12 hours, and observing and recording the infection and disease development condition every day. Through the reasonable culture and inoculation method and a reasonable culture medium formula, the identifying efficiency is improved, and large-batch screening is facilitated. |
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The method comprises the following steps: separating pathogens to be placed on a PDA culture medium platform to be cultured, washing with sterilized water, and filtering to obtain a spore suspending liquid; taking leaves of alfalfa, removing leafstalks, washing, conducting sterilization treatment, chopping into small blocks, putting into a 0.3% water agar culture medium containing 50 [mu]m/mL benzimidazole, and culturing for 4 days; selecting good leaves with the normal growth state, tiling on the culture medium, lightly stabbing the leaf surfaces with a pin, dripping the obtained spore suspending liquid at the stabbed parts, and conducting inoculation at the condition that the relative humidity is almost 100%, the temperature is 20 DEG C, and illumination is not provided, for 20 h; after the inoculation is finished, transferring the leaves into a growth box for culture, alternating the light intensity of 9000 lux and darkness every 12 hours, and observing and recording the infection and disease development condition every day. Through the reasonable culture and inoculation method and a reasonable culture medium formula, the identifying efficiency is improved, and large-batch screening is facilitated.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160106&DB=EPODOC&CC=CN&NR=105219835A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,781,886,25569,76552</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160106&DB=EPODOC&CC=CN&NR=105219835A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>QU SHANMIN</creatorcontrib><creatorcontrib>LIU XIANGPING</creatorcontrib><creatorcontrib>LI GUOLIANG</creatorcontrib><creatorcontrib>YANG ZHIMING</creatorcontrib><title>Method for identifying alfalfa brown spot</title><description>The invention discloses a method for identifying alfalfa brown spot. The method comprises the following steps: separating pathogens to be placed on a PDA culture medium platform to be cultured, washing with sterilized water, and filtering to obtain a spore suspending liquid; taking leaves of alfalfa, removing leafstalks, washing, conducting sterilization treatment, chopping into small blocks, putting into a 0.3% water agar culture medium containing 50 [mu]m/mL benzimidazole, and culturing for 4 days; selecting good leaves with the normal growth state, tiling on the culture medium, lightly stabbing the leaf surfaces with a pin, dripping the obtained spore suspending liquid at the stabbed parts, and conducting inoculation at the condition that the relative humidity is almost 100%, the temperature is 20 DEG C, and illumination is not provided, for 20 h; after the inoculation is finished, transferring the leaves into a growth box for culture, alternating the light intensity of 9000 lux and darkness every 12 hours, and observing and recording the infection and disease development condition every day. 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The method comprises the following steps: separating pathogens to be placed on a PDA culture medium platform to be cultured, washing with sterilized water, and filtering to obtain a spore suspending liquid; taking leaves of alfalfa, removing leafstalks, washing, conducting sterilization treatment, chopping into small blocks, putting into a 0.3% water agar culture medium containing 50 [mu]m/mL benzimidazole, and culturing for 4 days; selecting good leaves with the normal growth state, tiling on the culture medium, lightly stabbing the leaf surfaces with a pin, dripping the obtained spore suspending liquid at the stabbed parts, and conducting inoculation at the condition that the relative humidity is almost 100%, the temperature is 20 DEG C, and illumination is not provided, for 20 h; after the inoculation is finished, transferring the leaves into a growth box for culture, alternating the light intensity of 9000 lux and darkness every 12 hours, and observing and recording the infection and disease development condition every day. Through the reasonable culture and inoculation method and a reasonable culture medium formula, the identifying efficiency is improved, and large-batch screening is facilitated.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for identifying alfalfa brown spot |
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