Tritepenoidic acid active site of ganoderma lucidum, method for preparing tritepenoidic acid active site and application thereof

The invention discloses a tritepenoidic acid active site of ganoderma lucidum, a method for preparing the tritepenoidic acid active site and application of the tritepenoidic acid active site to preparing sleep quality improving healthcare products and mind tranquillizing medicine. The method includes smashing ganoderma lucidum medicinal materials to obtain dry powder, adding compound enzymes into the dry powder in water to carry out enzymatic hydrolysis and carrying out enzyme deactivation, cooling and pumping filtration to obtain enzymatic hydrolysis solution; adding the enzymatic hydrolysis solution into petroleum ether to extract the enzymatic hydrolysis solution, removing grease, adding ethyl acetate into the enzymatic hydrolysis solution to extract the enzymatic hydrolysis solution so as to obtain ethyl acetate extracts of the ganoderma lucidum; alkalizing the ethyl acetate extracts of the ganoderma lucidum, then washing the ethyl acetate extracts of the ganoderma lucidum until the ethyl acetate extracts of the ganoderma lucidum are neutral to obtain alkalized water layers, then acidifying the alkalized water layers and extracting the alkalized water layers by the aid of ethyl acetate to obtain crude ganoderma lucidum acid; separating the crude ganoderma lucidum acid by the aid of silica gel columns, eluting the crude ganoderma lucidum acid by the aid of chloroform and methanol systems in a gradient manner and carrying out thin-layer chromatography identification, filtering and drying by distillation to obtain the tritepenoidic acid active site of the ganoderma lucidum. The tritepenoidic acid active site, the method and the application have the advantage that the tritepenoidic acid active site of the ganoderma lucidum is subjected to enzymatic hydrolysis by the aid of the compound enzymes, can be obtained by the aid of steps of extraction, separation and the like and can be used for preparing the sleep quality improving healthcare products and the mind tranquillizing medicine.