Purification method of recombinant human vascular endothelial growth factor receptor-antibody fusion protein

Purification method of recombinant human vascular endothelial growth factor receptor-antibody fusion protein

The invention relates to production of a genetic engineering drug by a recombinant DNA technology, belongs to the biotechnical field, and particularly relates to a purification method of a recombinant human vascular endothelial growth factor receptor-antibody fusion protein, that is to say, a cell s...

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Hauptverfasser: SHI GAN, JIA GUODONG, XING TINGTING, DING RENZHI, CHEN JUNLIANG, ZHU PENGFEI, WANG QINGMIN, SUN LIXIA, SUN BO, ZHENG HUANLAN
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creator SHI GAN
JIA GUODONG
XING TINGTING
DING RENZHI
CHEN JUNLIANG
ZHU PENGFEI
WANG QINGMIN
SUN LIXIA
SUN BO
ZHENG HUANLAN
description The invention relates to production of a genetic engineering drug by a recombinant DNA technology, belongs to the biotechnical field, and particularly relates to a purification method of a recombinant human vascular endothelial growth factor receptor-antibody fusion protein, that is to say, a cell supernatant containing the recombinant human vascular endothelial growth factor receptor-antibody fusion protein is successively subjected to affinity chromatography, anion exchange chromatography, cation exchange chromatography, hydrophobic chromatography and desalination chromatography to obtain the high-quality recombinant human vascular endothelial growth factor receptor-antibody fusion protein. The purification method has the characteristics of low cost, high purity of the target protein, easily controlled technological process, easily large-scale enlarged production and the like.
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ORGANIC CHEMISTRY
PEPTIDES
title Purification method of recombinant human vascular endothelial growth factor receptor-antibody fusion protein
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