Preparation method for porcine circovirus type 2 CAP antigen
The invention provides a preparation method for a porcine circovirus type 2 CAP antigen. According to the design technique scheme of the method, the purpose of acquiring porcine circovirus type 2 sample particles is achieved, and the expression efficiency of porcine circovirus type 2 CAP protein is...
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| creator | GUO HUIJUAN LIANG WU LYU MAOJIE YANG BAOSHOU FU XUBIN |
| description | The invention provides a preparation method for a porcine circovirus type 2 CAP antigen. According to the design technique scheme of the method, the purpose of acquiring porcine circovirus type 2 sample particles is achieved, and the expression efficiency of porcine circovirus type 2 CAP protein is improved through a baculovirus expression system; in addition, gene modification is performed according to the preference of the baculovirus expression system to codon on the basis of natural expression genes of the porcine circovirus type 2 CAP protein, and therefore the expression efficiency of recombinant expression genes in the baculovirus expression system is significantly improved. On the basis, suitable purification conditions are matched for the baculovirus expression system and the self characteristics of the CAP protein; according to the purification method, on one hand, the higher purity yield is achieved, on the other hand, the product purified through the purification conditions can effectively form the virus particles (VLP) of the porcine circovirus type 2, and therefore the immunogenicity is guaranteed. |
| format | Patent |
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According to the design technique scheme of the method, the purpose of acquiring porcine circovirus type 2 sample particles is achieved, and the expression efficiency of porcine circovirus type 2 CAP protein is improved through a baculovirus expression system; in addition, gene modification is performed according to the preference of the baculovirus expression system to codon on the basis of natural expression genes of the porcine circovirus type 2 CAP protein, and therefore the expression efficiency of recombinant expression genes in the baculovirus expression system is significantly improved. On the basis, suitable purification conditions are matched for the baculovirus expression system and the self characteristics of the CAP protein; according to the purification method, on one hand, the higher purity yield is achieved, on the other hand, the product purified through the purification conditions can effectively form the virus particles (VLP) of the porcine circovirus type 2, and therefore the immunogenicity is guaranteed.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2015</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20151125&DB=EPODOC&CC=CN&NR=105087606A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,781,886,25569,76552</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20151125&DB=EPODOC&CC=CN&NR=105087606A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>GUO HUIJUAN</creatorcontrib><creatorcontrib>LIANG WU</creatorcontrib><creatorcontrib>LYU MAOJIE</creatorcontrib><creatorcontrib>YANG BAOSHOU</creatorcontrib><creatorcontrib>FU XUBIN</creatorcontrib><title>Preparation method for porcine circovirus type 2 CAP antigen</title><description>The invention provides a preparation method for a porcine circovirus type 2 CAP antigen. According to the design technique scheme of the method, the purpose of acquiring porcine circovirus type 2 sample particles is achieved, and the expression efficiency of porcine circovirus type 2 CAP protein is improved through a baculovirus expression system; in addition, gene modification is performed according to the preference of the baculovirus expression system to codon on the basis of natural expression genes of the porcine circovirus type 2 CAP protein, and therefore the expression efficiency of recombinant expression genes in the baculovirus expression system is significantly improved. 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According to the design technique scheme of the method, the purpose of acquiring porcine circovirus type 2 sample particles is achieved, and the expression efficiency of porcine circovirus type 2 CAP protein is improved through a baculovirus expression system; in addition, gene modification is performed according to the preference of the baculovirus expression system to codon on the basis of natural expression genes of the porcine circovirus type 2 CAP protein, and therefore the expression efficiency of recombinant expression genes in the baculovirus expression system is significantly improved. On the basis, suitable purification conditions are matched for the baculovirus expression system and the self characteristics of the CAP protein; according to the purification method, on one hand, the higher purity yield is achieved, on the other hand, the product purified through the purification conditions can effectively form the virus particles (VLP) of the porcine circovirus type 2, and therefore the immunogenicity is guaranteed.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Preparation method for porcine circovirus type 2 CAP antigen |
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